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品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB, ICC Rb Serum Polyclonal Antibody 描述 产品目录编号 ABE262 Replaces 04-232 描述 Anti-HDAC4 Antibody Alternate Names - HD4
- Histone deacetylase 4
背景信息 Histone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Transcription is controlled in part by the dynamic acetylation and deacetylation of histone proteins. Histone deacetylases (HDACs) are recruited to specific promoters or chromosomal domains by association with DNA-binding proteins. HDAC4 is a histone deacetylase which deacetylates lysines on the core histones. HDAC4 accumulates in the nuclei of myotubes, suggesting involvement in muscle differentiation. HDAC4 does not bind DNA directly but interacts with the myocyte enhancer factors MEF2A, MEF2C, and MEF2D. HDAC4 also interacts with the breast cancer associated transcriptional repressor PLU-1/JARID1B, the Nr2c1/TR2 nuclear receptor, as well as the 14-3-3 chaperone protein, in a phosphorylation dependent manner. 产品信息 格式 Serum 控制 - HepG2 cell lysate
演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Use Anti-HDAC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP, ChIP to detect HDAC4 also known as Histone deacetylase 4, HD4. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HDAC4 in HeLa and A431 cells.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of human HDAC4. 表位 C-terminus 宿主 Rabbit 特异性 This antibody recognizes HDAC4 at the C-terminus. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006028
Entrez基因汇总 Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]. 基因符号 - HDAC4
纯化方法 Unpurified UniProt编号 - P56524
UniProt汇总 FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
CATALYTIC ACTIVITY: Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
SUBUNIT: Interacts with HDAC7 (By similarity). Homodimer. Homodimerization via its N-terminal domain. Interacts with MEF2C, AHRR, and NR2C1. Interacts with a 14-3-3 chaperone protein in a phosphorylation dependent manner. Interacts with BTBD14B (By similarity). Interacts with JARID1B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note= Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
PTM: Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.
Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.产品使用声明 质量保证 Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected HDAC4 on 10 µg of HepG2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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