merck millipore,默克密理博,MABE196,Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal

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merck millipore,默克密理博,MABE196,Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal
产品名称：Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal
产品型号：MABE196
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,MABE196,Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABE196,Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	FC, WB	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABE196
描述	Anti-Rb2/p130 Antibody, clone EP2141Y, rabbit monoclonal
Alternate Names	retinoblastoma-like protein 2 retinoblastoma-like 2 (p130) Retinoblastoma-related protein 2 130 kDa retinoblastoma-associated protein
背景信息	p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G₀ restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G₀ cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).

产品信息
格式	Diluted Culture Supernatant
控制	HeLa and A431 cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Flow Cytometry Western Blotting
应用说明	Flow Cytometry Analysis: 1:20 dilution from a representative lot was used in FC. Immunohistochemistry Analysis: 1:50-100 dilution from a representative lot detected Rb2/p130 in human breast carcinoma tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to human Rb2/p130 near the C-terminus.
表位	Near C-terminus
克隆	EP2141Y
宿主	Rabbit
特异性	This antibody recognizes Rb2/p130 near the C-terminus.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human.
抗体类型	Monoclonal Antibody
基因符号	RB2 Rb2 p130 RBR-2 FLJ26459 PRB2 P130 pRb2
纯化方法	Unpurified
UniProt编号	Q08999
UniProt汇总	FUNCTION: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. SUBUNIT STRUCTURE: Interacts with AATF. Interacts with SUV420H1, SUV420H2 and USP4 By similarity. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1. SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: G0-restricted expression. PTM: During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. SEQUENCE SIMILARITES: Belongs to the retinoblastoma protein (RB) family.

产品使用声明
质量保证	Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 1:500 dilution of this antibody detected Rb2/p130 in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.