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merck millipore,默克密理博,MABE18,Anti-PARP-2 Antibody, clone 4G8
产品名称：Anti-PARP-2 Antibody, clone 4G8
产品型号：MABE18
Anti-PARP-2 Antibody, clone 4G8 is a Mouse Monoclonal Antibody for detection of PARP-2 also known as poly (ADP-ribose) polymerase 2 or poly(ADP-ribose) synthetase & has been validated in WB, ELISA, IP More>>
merck millipore,默克密理博,MABE18,Anti-PARP-2 Antibody, clone 4G8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MABE18 描述 Anti-PARP-2 Antibody, clone 4G8 Alternate Names - ARTD1
- Poly [ADP-ribose] polymerase 1
- PARP-1
- NAD(+) ADP-ribosyltransferase 1
- ADPRT 1
- Poly[ADP-ribose] synthase 1
背景信息 Poly(ADP-ribose) polymerase (PARP) is a highly conserved nuclear enzyme present in higher eukaryotes. This gene is a member of a family of enzymes that synthesize and transfer ADP-ribose polymers to protein to modify their functional properties. A key role of PARP in the cell is mediating the repair of single-strand DNA nicks. PARP-2 forms homodimers and hetero dimers with PARP-1 in the base excision repair (BER) pathway. To mediate repair PARP interacts with DNA and recruits DNA repair protein XRCC1, DNA ligase III, DNA polymerase beta and a kinase to the site of damage. As a marker for apoptosis, it has been shown that PARP cleavage mediated by caspase-3 activity occurs at the onset of apoptosis, and inhibition of PARP cleavage attenuates apoptosis in vitro. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-PARP-2 Antibody, clone 4G8 is a Mouse Monoclonal Antibody for detection of PARP-2 also known as poly (ADP-ribose) polymerase 2 or poly(ADP-ribose) synthetase & has been validated in WB, ELISA, IP & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
- ELISA
应用说明 Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Maeda, Y., et al. (2006). JBC. 281(14):9600-9606.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH.
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (von Kobbe, C., et al. (2004). Nucleic Acids Research. 32(13):4003-4014.)生物信息 免疫原品种 Recombinant protein corresponding to mouse PARP-2. 表位 Unknown 克隆 4G8 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PARP-2. 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_033762
Entrez基因汇总 This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of
catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly
(ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain
of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential
DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two
alternatively spliced transcript variants encoding distinct isoforms have been found. (provided by RefSeq)
基因符号 - ADPRTL3
- pADPRT-2
- PARP-2
- hPARP-2
- ADPRT2
- ADPRTL2
纯化方法 Protein G purfied UniProt编号 - O88554
UniProt汇总 FUNCTION: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
CATALYTIC ACTIVITY: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
SUBUNIT STRUCTURE: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP1, POLB and LIG3. Homo- and heterodimer with PARP1.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Widely expressed; the highest levels were in testis followed by ovary. Expression is correlated with proliferation, with higher levels occurring during early fetal development and organogenesis and in the highly proliferative cell compartments of adult.
DEVELOPMENTAL STAGE: At stage E12.5, expressed at high level in the developing liver and kidneys. At E18.5, preferentially expressed in the thymus and in regions of the nervous system. Within the developing trunk, preferential expression persisted in the liver and became restricted to the cortical region of the kidney, spleen, adrenal gland, and to stomach and intestinal epithelia. From E14.5 to E18.5, as well as in the adult, expressed at the highest level in thymus. Expression is particularly high in the subcapsular zone of the thymus where immature lymphocytes proliferate.
INDUCTION: By high levels of DNA-damaging agents.
PTM: Poly-ADP-ribosylated by PARP1.
Acetylation reduces DNA binding and enzymatic activity.
SEQUENCE SIMILARITIES: Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
SEQUENCE CAUTION: The sequence AAC25415.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PARP-2 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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