- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABC631 描述 Anti-KLF4 Antibody, clone 1E6 Alternate Names - Gut-enriched krueppel-like factor
- Krueppel-like factor 4
- Epithelial zinc finger protein EZF
背景信息 Krueppel-like factor 4 (KLF4), which is also known as epithelial zinc finger protein (EZF) and gut-enriched krueppel-like factor (GKLF), is a developmentally regulated transcription factor that plays an important role in maintaining the pluripotent state in embryonic stem cells (ESCs) and is used as a marker of the pluripotent state for cells in culture. It is required for the differentiation of epithelial cells and the establishment of the barrier function of the skin, and KLF4 is also expressed during skeletal and kidney development. KLF4 is highly expressed in the skin, lung, and intestine along the epithelial border. This nuclear localized protein can act as both a tumor suppressor and activator depending upon its environment, but its most prominent function is growth suppression. Interestingly, increased expression of KLF4 leads to an increase in expression of P21WAF1/CIP1 which is an important checkpoint protein that inhibits cell cycle progression and is essential in mediating the cell cycle arrest at both the G1/S and G2/M boundaries. Additionally, KLF4 mediates the normal functions of the tumor suppressor p53 as well. Mutations thus in KLF4 lead to unchecked growth and tumor formation, and because of its expression and involvement within the gut, KLF4 is often mutated in many colon cancer cell lines and the lack of KLF4 expression is correlated with colorectal cancer pathogenesis. Elevated expression of KLF4 gene expression has also been associated with gastric cancer, breast cancer, and squamous cell carcinomas. EMD-Millipore’s Anti-KLF4 mouse monoclonal antibody, clone 1E6, has been tested in western blot against recombinant hKLF4 expressed cell lysates and in immunocytochemistry on paraffin embedded human lung and colon cancer tissue. The clone has also been tested in fluorescent immunocytochemistry on the human esophageal squamous carcinoma cell line Eca109 in culture. 产品信息 格式 Ascites 控制 - Human lung cancer and colon tissues
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Detect KLF4 using this mouse monoclonal antibody, Anti-KLF4 Antibody, clone 1E6 validated for use in IHC & western blotting. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected KLF4 in ECA109 cells.
Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected KLF4 in transfected HEK293 cell lysates.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant fragment of human KLF4 expressed in E. coli. 克隆 1E06 宿主 Mouse 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_004226
基因符号 - KLF4
- EZF
- GKLF
纯化方法 Unpurified UniProt编号 - O43474
产品使用声明 质量保证 Evaluated by Immunohistochemistry in human lung cancer and colon tissues.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected KLF4 in human lung cancer and colon tissues.
Optimal working dilutions must be determined by end user.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,MAB1275,Anti-HLA-ABC Common Antibody, clone 22.63.4
下一件merck millipore产品：merck millipore,默克密理博,420320,KT5720 - CAS 108068-98-0 - Calbiochem
相关产品
merck millipore,默克密理博,219494,Cdk/CKI Inhibitor, (R)-DRF053 - CAS 1186647-87-9 - Calbiochem
merck millipore,默克密理博,MVCPN0025,MultiScreen High Volume Collection Plates, opaque, non-sterile
merck millipore,默克密理博,AQ106P,Rabbit Anti-Goat IgG Antibody, F(ab') 2, HRP conjugate
merck millipore,默克密理博,ABN42,Anti-GADD45B Antibody
merck millipore,默克密理博,MAB1275,Anti-HLA-ABC Common Antibody, clone 22.63.4
merck millipore,默克密理博,420320,KT5720 - CAS 108068-98-0 - Calbiochem
merck millipore,默克密理博,ABE501,Anti-phospho IRF3 (ser386) Antibody
merck millipore,默克密理博,14-531,EGFR Protein, active, 10 µg
merck millipore,默克密理博,AB19027,Anti-ADAM 17 Antibody
merck millipore,默克密理博,23-027M,b-catenin Protein, activated, 250 µg