merck millipore,默克密理博,MABC175,Anti-LC3A Antibody, clone EPR1754, rabbit monoclonal

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merck millipore,默克密理博,MABC175,Anti-LC3A Antibody, clone EPR1754, rabbit monoclonal
产品名称：Anti-LC3A Antibody, clone EPR1754, rabbit monoclonal
产品型号：MABC175
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect LC3A using this Anti-LC3A Antibody, clone EPR More>>

merck millipore,默克密理博,MABC175,Anti-LC3A Antibody, clone EPR1754, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABC175,Anti-LC3A Antibody, clone EPR1754, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	MABC175
描述	Anti-LC3A Antibody, clone EPR1754, rabbit monoclonal
Alternate Names	microtubule-associated proteins 1A/1B light chain 3A microtubule-associated protein 1 light chain 3 alpha Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A2 MAP1 light chain 3-like protein 1 MAP1A/MAP1B LC3 A MAP1A/MAP1B light chain 3 A MAP1A/1B light chain 3 A microtubule-associated proteins 1A/1B light chain 3
背景信息	Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing glycerol, BSA, and sodium azide.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect LC3A using this Anti-LC3A Antibody, clone EPR1754, Rabbit Monoclonal validated for use in Western Blotting, Flow Cytometry.
主要应用	Flow Cytometry Western Blotting
应用说明	Flow Cytometry Analysis: A 1:40 dilution from a representative lot was used to detect LC3A in FC.

生物信息
免疫原品种	Synthetic peptide corresponding to the N-terminus of human LC3A.
克隆	EPR1754
宿主	Rabbit
特异性	This antibody recognizes the N-terminus of LC3A.
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with Human, Rat, and Mouse. Species cross reactivity is based on WB analysis.
抗体类型	Monoclonal Antibody
Entrez基因编号	Q9H492
基因符号	MAP1ALC3 MAP1BLC3 ATG8E LC3 LC3A OTTHUMP00000030696 OTTHUMP00000030697 OTTHUMP00000030698
纯化方法	Unpurified
UniProt编号	Q9H492
UniProt汇总	FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT STRUCTURE: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. SEQUENCE SIMILARITIES: Belongs to the MAP1 LC3 family.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa cell lysate Western Blot Analysis: A 1:500 dilution of this antibody was used to detect LC3A in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.