merck millipore,默克密理博,MABC123,Anti-Angiotensinogen Antibody, clone EPR2931, rabbit monoclonal

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merck millipore,默克密理博,MABC123,Anti-Angiotensinogen Antibody, clone EPR2931, rabbit monoclonal
产品名称：Anti-Angiotensinogen Antibody, clone EPR2931, rabbit monoclonal
产品型号：MABC123
Anti-Angiotensinogen Antibody, clone EPR2931, Rabbit is an antibody against Angiotensinogen for use in WB, IC & IP.

merck millipore,默克密理博,MABC123,Anti-Angiotensinogen Antibody, clone EPR2931, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABC123,Anti-Angiotensinogen Antibody, clone EPR2931, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABC123
描述	Anti-Angiotensinogen Antibody, clone EPR2931, rabbit monoclonal
Alternate Names	serine (or cysteine) proteinase inhibitor angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Serpin A8 angiotensin II angiotensin I angiotensinogen pre-angiotensinogen alpha-1 antiproteinase, antitrypsin
背景信息	Angiotensinogen is a precursor of angiotensin-1,2, and 3; as a member of the serpin family, it is expressed by the liver and secreted in plasma. In response to lowered blood pressure, renin cleaves angiotensinogen and produce angiotensin-1 (1). Angiotensin-converting enzyme (ACE) further removes a dipeptide to produce the physiologically active peptide angiotensin-2. Angiotensin-2 is involved in regulating mineral balance and volume of body fluids, while angiotensin-3 stimulates aldosterone release (2). Defects in angiotensinogen have been linked to renal tubular dygenesis (RTD) and susceptibility to essential hypertension (3).

产品信息
格式	Diluted Culture Supernatant
控制	Fetal artery, fetal heart, fetal liver, HepG2, 293T, and human plasma lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-Angiotensinogen Antibody, clone EPR2931, Rabbit is an antibody against Angiotensinogen for use in WB, IC & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected Angiotensinogen in HepG2 cells. Immunoprecipitation Analysis: A 1:10-100 dilution from a representative lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to human Angiotensinogen.
克隆	EPR2931
宿主	Rabbit
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
基因符号	FLJ92595 SERPINA8 FLJ97926 ANHU
纯化方法	Unpurified
UniProt编号	P01019
UniProt汇总	FUNCTION: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3 stimulates aldosterone release. Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 By similarity. Has vasodilator and antidiuretic effects By similarity. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets By similarity. SUBUNIT STRUCTURE: During pregnancy, exists as a disulfide-linked 2:2 heterotetramer with the proform of PRG2 and as a complex (probably a 2:2:2 heterohexamer) with pro-PRG2 and C3dg. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICITY: Expressed by the liver and secreted in plasma. PTM: Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. INVOLVEMENT IN DISEASE: Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). SEQUENCE SIMILARITIES: Belongs to the serpin family. CAUTION: It is uncertain whether Met-1 or Met-10 is the initiator.

产品使用声明
质量保证	Evaluated by Western Blot in Fetal artery, fetal heart, fetal liver, HepG2, 293T, and human plasma lysates. Western Blot Analysis: A 1:1,000-10,000 dilution of this antibody detected Angiotensinogen in Fetal artery, fetal heart, fetal liver, HepG2, 293T, and human plasma lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.