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merck millipore,默克密理博,MAB88051,Anti-Heat Shock Protein 27 Antibody, clone G3.1
产品名称：Anti-Heat Shock Protein 27 Antibody, clone G3.1
产品型号：MAB88051
Anti-Heat Shock Protein 27 Antibody, clone G3.1 is an antibody against Heat Shock Protein 27 for use in ELISA, IF, WB, IH, IH(P).
merck millipore,默克密理博,MAB88051,Anti-Heat Shock Protein 27 Antibody, clone G3.1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Chp, H, Mk IHC, ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB88051 Replaces MAB3512 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Heat Shock Protein 27 Antibody, clone G3.1 Alternate Names - HSP27
背景信息 ANTIGEN DISTRIBUTION:
Hsp27 is identical to estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are hsp27 positive, especially those that are positive for ER and/or PgR. Hsp 27 is also complicated in drug resistance. MAB88051 is excellent for biochemical and histochemical applications.产品信息 格式 Purified 控制 - POSITIVE CONTROL: BT474 or MDA-MB-453 cells and breast carcinoma.
演示 Purified immunoglobulin presented as a liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 Anti-Heat Shock Protein 27 Antibody, clone G3.1 is an antibody against Heat Shock Protein 27 for use in ELISA, IF, WB, IH, IH(P). 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Immunohistochemistry (frozen & formalin/paraffin): 1-2 μg/mL. 30-60 minutes at room temperature. Staining of
formalin fixed tissue sections requires boiling the tissue
sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes
followed by cooling at room temperature for 20 minutes. Both nuclear and cytosolic staining will be observed.
Western blot: 1-2 μg/mL. 2hrs RT; simple buffers without milk as cross reactivity with bovine HSP's may occur.
Immunofluorescence: 4% PFA fixed tissues; permabilize with 0.1% triton X-100 in block: 1:50-1:200.
ELISA (capture antibody; use goat anti-mouse Fc coating antibody, then coat with G3.1, 0.1-2μg/mL).
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. 克隆 G3.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes an estrogen-regulated protein of 24-27 kDa which is identified as hsp27. Hsp27 was recently found to be identical to the estrogen induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. Hsp27 has also been implicated in drug resistance in cancer cells.
CELLULAR LOCALIZATION:
Cytoplasmic / nuclear.同种型 IgG1 品种反应性 ChimpanzeeHumanMonkey 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001540.2
基因符号 - SRP27
- HSPB1
- Hs.76067
- HSP27
- Hsp25
- HspB1
- HSP28
- CMT2F
- HS.76067
- DKFZp586P1322
- HMN2B
非反应性品种 - Rat
- Mouse
UniProt编号 - P04792
UniProt汇总 FUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain material at 2-8°C for up to 12 months from date of receipt. 包装信息 数量 50 µg
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