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品种反应性 主要应用 宿主 格式 抗体类型 H IF M Purified Monoclonal Antibody 描述 产品目录编号 MAB810-KC 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR) 概述 This product is intended for use by IVD manufacturers. Alternate Names - CMV
背景信息 Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours (see figure below). After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication. 产品信息 格式 Purified 控制 - Cytomegalovirus Control Slides, Catalogue Number 5027-5
演示 Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. 应用 主要应用 - Immunofluorescence
应用说明 ELISA 1:800
Western Blot: 1:200. Recognizes approximately a 70kDa band.
Immunofluorescence: 1:200-1:400.
Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.
Works on paraffin embedded tissue sections.
FACS: See online protocol.
Final working dilutions and protocols must be determined by end user.生物信息 免疫原品种 Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC). 克隆 8B1.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle. 同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody 纯化方法 Protein A purfied 产品使用声明 销售限制 This product is only available for sale in the United States and Canada. 使用声明 - Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 Bulk packaging. Please inquire.
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