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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 ABS71 描述 Anti-GEF-H1 Antibody Alternate Names - Guanine nucleotide exchange factor H1
- Rho/Rac guanine nucleotide exchange factor (GEF) 2
- Rho/Rac guanine nucleotide exchange factor 2
- Proliferating cell nucleolar antigen p40
- Microtubule-regulated Rho-GEF
背景信息 Guanine nucleotide exchange factor H1 (GEF-H1) is a guanine nucleotide exchange factor containing 3 characteristic domains; a DH (DBL-homology) domain that functions to interact with and promote GTP loading on RhoA, a pleckstrin-homology (PH) domain that participates in the binding and targeting of microtubules to tight junctions, and a phorbol-ester/DAG-type zinc finger. GEF-H1 functions to promote the GDP to GTP exchange for the activation of Rho-GTPases. Various studies have suggested that GEF-H1 may have several diverse roles such as; dendritic spine motility, cell motility and polarization, cell cycle regulation, antigen presentation, epithelial barrier permeability, leukemic cell differentiation, cancer, and accelerated cell proliferation within tumor cells. Currently, there are three known isoforms resulting from alternative splicing. 产品信息 格式 Purified 控制 - HEK293 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-GEF-H1 Antibody is an antibody against GEF-H1 for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 2 µg/mL of this antibody was shown to detect GEF-H1 on 10 µg of L6 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human GEF-H1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes GEF-H1. 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004714
Entrez基因汇总 Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq]. 基因符号 - GEFH1
- GEF
- GEF-H1
- LFP40
- P40
纯化方法 Protein G purfied UniProt编号 - Q92974
UniProt汇总 FUNCTION: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in Ref.1 (Ren Y., et al. (1998). J. Biol. Chem. 273:34954-34960.). May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases.
SUBUNIT STRUCTURE: Interacts with 14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4, AURKA/STK6 and MAPK1. Interacts with RHOA and RAC1.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction › tight junction (By similarity). Golgi apparatus. Cytoplasm › cytoskeleton › spindle. Note: Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization.
DOMAIN: The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.
PTM: Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules.
SEQUENCE SIMLARITIES: Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
SEQUENCE CAUTION: The sequence AAC97383.1 differs from that shown. Reason: Sequence differs at a large extent from the sequence shown in the paper.
The sequence AAH20567.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA33634.1 differs from that shown. Reason: Frameshift at positions 887 and 984.产品使用声明 质量保证 Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected GEF-H1 on 10 µg of HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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