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merck millipore,默克密理博,AB5194-200UL,Anti-Potassium Channel Kv4.3 Antibody
产品名称：Anti-Potassium Channel Kv4.3 Antibody
产品型号：AB5194-200UL
Detect Potassium Channel Kv4.3 using this Anti-Potassium Channel Kv4.3 Antibody validated for use in IH & WB. Less<<
merck millipore,默克密理博,AB5194-200UL,Anti-Potassium Channel Kv4.3 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5194-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Potassium Channel Kv4.3 Antibody 产品信息 格式 Affinity Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 Detect Potassium Channel Kv4.3 using this Anti-Potassium Channel Kv4.3 Antibody validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry on rat brain sections.
Western blot: 1:200 using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
PROTOCOL FOR IMMUNOHISTOCHEMISTRY
Sacrifice and tissue processing:
Rats are deeply anesthetized with pentobarbital sodium (Pental). Brain is fixed by transcardial perfusion, first with 50 mL of phosphate buffered saline (0.02M PBS, pH 7.4) containing heparin (5U/ml), then with 220 mL of ice-cold 4% paraformaldehyde in 0.1M PBS, pH 7.5 containing sucrose 4%. Brain is cut in coronal blocks and further fixed by immersion in the same fixative, refrigerated, for 1-2 hours. Brain blocks are transferred to 10% sucrose in 0.1M PBS and sectioned in a cryostat within 3 days. Brain sections, 30 μm thick, are floated in 0.1M PBS and then preserved in a cryopreservation buffer at -20°C. The cryopreservation buffer contains 40% ethylene glycol and 1% polyvinylpyrrolidone in 0.1M potassium acetate buffer, pH 6.5.
Staining procedure:
1. Floating sections are rinsed in 0.02M PBS, 2x 5 minutes.
2. Endogenous peroxidase activity is quenched by incubation with 0.2 % hydrogen peroxide in 0.1M phosphate buffer pH 7.3 containing 0.2% Triton X-100 for 25 minutes at room temperature.
3. Sections are rinsed in 0.02M PBS, 2x 5 minutes.
4. Sections are incubated with the primary antiserum in a medium containing 0.3% Triton X-100, 0.05% Tween 20, 4% normal donkey serum (NDS), for 1 hour at room temperature and then overnight refrigerated.
5. Sections are rinsed in 0.02M PBS, containing 4% NDS, 2x 5 minutes.
Sections are incubated with biotinylated donkey anti-rabbit (from Chemicon USA, catalog number AP182B) diluted 1:400 in 0.02M PBS, containing 0.3% Triton X-100, 0.05% Tween 20, and 4% NDS, for 1 hour at room temperature and then overnight refrigerated.
1. Sections are rinsed in 0.02M PBS containing 4% NDS, 2x 5 min.
2. Sections are incubated with streptavidin-peroxidase, appropriately diluted in 0.02M PBS, for 45 minutes at room temperature.
3. Sections are rinsed in 0.02M PBS, 3x 5 minutes.
Color development:
1. Sections are incubated with a solution of diaminobenzidine at the concentration of 0.0125% and containing 0.05% nickel ammonium sulfate for 10 minutes at room temperature.
2. Sections are transferred to the same DAB solution but with added hydrogen peroxide at a final concentration of 0.0015%. Duration of incubation should be adjusted by the end user.
3. Sections are rinsed in 0.02M PBS, 4x 10 minutes.
4. Sections are mounted on glass slides (gelatinized or coated by any other type of adhesive material) and allowed to dry.
5. Sections are dehydrated in ascending series of ethanol concentrations (70%, 90%, 100%, 5 minutes in each), delipidated in xylene (10 minutes) and coverslipped in Permount (or any other xylene diluted adhesive).生物信息 免疫原品种 Purified peptide from rat Kv4.3 (amino acids 451-467). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes a full length Kv4.3protein. Does not cross react with any other potassium channel antigens tested so far.
SPECIES REACTIVITIES: It is expected that the antibody will react with rabbit (17/17) and mouse (16/17) due to sequence homology. Other species have not been tested.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_172198.1
- NM_004980.3
Entrez基因汇总 Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene. 基因符号 - MGC142035
- KCND3
- OTTHUMP00000063948
- KV4.3
- KCND3S
- KCND3L
- KSHIVB
- Kv4.3
- MGC142037
UniProt编号 - Q9UK17
UniProt汇总 FUNCTION: SwissProt: Q9UK17 # Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
SIZE: 655 amino acids; 73451 Da
SUBUNIT: Homotetramer or heterotetramer with KCND1 and/or KCND2. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4 (By similarity). Interacts with KCNE1, KCNE2, SCN1B and KCNAB1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.
DOMAIN:SwissProt: Q9UK17 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. D (Shal) subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 200 µL
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