merck millipore,默克密理博,MAB5412,Anti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, IHC	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB5412
Replaces	AB5968
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1
Alternate Names	Indoleamine-pyrrole 2,3-dioxygenase
背景信息	Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

产品信息
格式	Purified
控制	IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.
演示	Purified mouse monoclonal IgG3 in buffer containing 0.02 M phosphate, 0.25 M NaCl, 0.1% NaN3, pH 7.6.

应用
应用	Anti-Indoleamine 2, 3-dioxygenase Antibody, clone 10.1 is an antibody against Indoleamine 2 for use in IH & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunohistochemistry: A previous lot of this antibody worked on lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody. High temperature antigen retrieval is recommended. Western blot: Recognizes a 42-45kDa band in IFN-gamma treated human HeLa cell lines. Mouse IDO runs slightly smaller in blots than human IDO. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Amino acids 78-184 of human IDO fused to GST.
克隆	10.1
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Reacts with Indolamine 2,3-dioxygenase (IDO). Note IDO is usually expressed in response to various stimuli. A good positive control is HeLa cells stimulated with IFN-gamma.
同种型	IgG3
品种反应性	MouseHuman
Species Reactivity Note	Expected to react with mouse and rat based on sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002164.4
Entrez基因汇总	Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]
基因符号	EC 1.13.11.52 IDO INDO
纯化方法	Protein A purfied
UniProt编号	P14902
UniProt汇总	FUNCTION: SwissProt: P14902 # Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. COFACTOR: Binds 1 heme group per subunit. SIZE: 403 amino acids; 45326 Da SIMILARITY: SwissProt: P14902 ## Belongs to the indoleamine 2,3-dioxygenase family.

产品使用声明
质量保证	Routinely evaluated by Western Blot on untreated and IFNgamma treated HeLa lysates. Western blot: 1:500 dilution of this lot detected IDO on 10 μg of untreated and IFN gamma treated HeLa lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MAB5412,Anti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1

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