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merck millipore,默克密理博,MAB5406B,Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
产品名称：Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
产品型号：MAB5406B
This Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67.
merck millipore,默克密理博,MAB5406B,Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC M Biotin Monoclonal Antibody 描述 产品目录编号 MAB5406B 描述 Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate Alternate Names - Glutamate decarboxylase 67 kDa isoform
- Glutamate decarboxylase 1
- 67 kDa glutamic acid decarboxylase
- GAD-67
背景信息 Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS. 产品信息 格式 Biotin 控制 - Rat cortex tissue
演示 Purified mouse monoclonal IgG2a conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 应用 This Anti-GAD67 Antibody, clone 1G10.2, Biotin Conjugate is validated for use in IHC for the detection of GAD67. 主要应用 - Immunohistochemistry
应用说明 Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAD67 in rat E18 primary cortex cells. 生物信息 免疫原品种 Recombinant GAD67 protein 克隆 1G10.2 宿主 Mouse 特异性 Reacts with GAD67. No detectable cross reactivity with GAD65 by Western blot on rat brain lysate. 同种型 IgG2a 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Rat. Predicted to react with Human and Mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000808
基因符号 - GAD67
- GAD1
- GAD
纯化方法 Protein A purified UniProt编号 - Q99259
UniProt汇总 FUNCTION: Catalyzes the production of GABA.
CATALYTIC ACTIVITY: L-glutamate = 4-aminobutanoate + CO2.
COFACTOR: Pyridoxal phosphate.
SUBUNIT STRUCTURE: Homodimer.
TISSUE SPECIFICITY: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
INVOLVEMENT IN DISEASE: Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
SEQUENCE SIMILARITIES: Belongs to the group II decarboxylase family.产品使用声明 质量保证 Evaluated by Immunohistochemistry in rat cortex tissue.
Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected GAD67 in rat cortex tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µL
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