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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, B, H WB, ELISA, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5328 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-RAGE Antibody, clone DD/A11 or A11 Alternate Names - Receptor for Advanced Glycosylation End Products.
背景信息 RAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimer's disease, and this interaction results in oxidative stress leading to neuronal degeneration. 产品信息 格式 Purified 控制 - Lung tissue, mouse brain lysate.
演示 Purified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide. 应用 应用 Anti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections.
Immunocytochemistry:
A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody.
ELISA:
A previous lot was shown to work on direct ELISA.
Immunoblotting:
Recognizes a band of ~48kDa in bovine lung extract.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Recombinant truncated extracellular part of RAGE produced in E. coli. 表位 Extracellular 克隆 DD/A11 or mAbA11 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 RAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE. 同种型 IgG2a 品种反应性 MouseBovineHuman Species Reactivity Note Mouse. Expected to react with rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_014226.1
基因符号 - EC 2.7.11.22
- RAGE
- RAGE-1
- MOK
- RAGE1
纯化方法 Protein A purfied UniProt编号 - Q15109
UniProt汇总 FUNCTION: SwissProt: Q15109 # Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Receptor for amyloid beta peptide.
SIZE: 404 amino acids; 42803 Da
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
TISSUE SPECIFICITY: Endothelial cells.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q15109 # Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot:
1:500 dilution of this lot detected RAGE on 10 μg of Mouse Brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,70189,pT7Blue Perfectly Blunt Cloning Kit - Novagen
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