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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5302 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Acetylcholine Antibody 产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid containing 0.005% sodium merthiolate and 0.05% sodium azide. 应用 应用 This Anti-Acetylcholine Antibody is validated for use in IH for the detection of Acetylcholine. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry: 1:250-1:2,500 using free floating sections by the PAP technique on rat cholinergic areas. Tissues should be fixed with a mixture of nitrobenzyl alcohol and gluteraldehyde (Chagnaud, 1989). Gluteraldehyde is required for staining; not recommended for formalin or paraformaldehyde fixations without gluteraldehyde.
ELISA: 1:1,000-1:5,000
Immunocytochemistry: 1:1,000-1:5,000
Western Blot: 1:1,000-1:2,000
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Choline-Glutaric anhydride-BSA. 宿主 Mouse 特异性 Acetylcholine. By immunohistochemistry on rat basal forbrain and spinal chord sections, the antibody specifically stains nerve cell bodies and fibres (Chagnaud, 1989).
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
Choline-GA-BSA 1
Choline-AA-BSA 1/536
Choline-SA-BSA 1/2,500
Farnesol-BSA 1/>50,000
Retinoic acid-BSA 1/>50,000
Myristic acid-BSA 1/>50,000
Acetylcholine 1/>50,000
Choline 1/>50,000
Abbreviations:
(GA) Glutaric anhydride
(AA) Adipic acid
(SA) Succinic acid
(BSA) Bovine Serum Albumin同种型 IgG2a 品种反应性 Rat 抗体类型 Monoclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 100 µL
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