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merck millipore,默克密理博,AB3561,Anti-phospho HSP27 (pS82) Antibody
产品名称：Anti-phospho HSP27 (pS82) Antibody
产品型号：AB3561
Anti-phospho HSP27 (pS82) Antibody detects level of phospho HSP27 (pS82) & has been published & validated for use in WB.
merck millipore,默克密理博,AB3561,Anti-phospho HSP27 (pS82) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3561 Replaces 04-448 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-phospho HSP27 (pS82) Antibody 背景信息 Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal α-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex. 产品信息 格式 Purified 控制 - HeLa cells treated with TNF-α.
演示 Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide. 应用 应用 Anti-phospho HSP27 (pS82) Antibody detects level of phospho HSP27 (pS82) & has been published & validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human HSP27 that contains serine 82. 宿主 Rabbit 特异性 Human HSP27. This antibody does not detect endogenous mouse HSP25 [pS86] protein in extracts of NIH3T3 cells treated with anisomycin; however, interaction with the human HSP27 protein is inhibited by the phosphopeptide used to generate the mouse HSP25 [pS86] antibody. 品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001540.2
基因符号 - SRP27
- HSPB1
- HspB1
- HS.76067
- HMN2B
- Hs.76067
- HSP27
- Hsp25
- HSP28
- CMT2F
- DKFZp586P1322
修改 - Phosphorylation
UniProt编号 - P04792
UniProt汇总 FUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient. 包装信息 数量 100 µL
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