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merck millipore,默克密理博,HTS065M,ChemiSCREEN™ Membrane Preparation Recombinant Human MT1 Melatonin Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human MT1 Melatonin Receptor
产品型号：HTS065M
Human MT1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS065M,ChemiSCREEN™ Membrane Preparation Recombinant Human MT1 Melatonin Receptor
- 产品介绍
- merck millipore,默克密理博,HTS065M,ChemiSCREEN™ Membrane Preparation Recombinant Human MT1 Melatonin Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 MT1 描述 产品目录编号 HTS065M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human MT1 Melatonin Receptor 概述 Full-length human MTNR1A cDNA encoding MT1 背景信息 Melatonin (5-methoxy-acetyltryptamine) is a vertebrate hormone synthesized and secreted in a circadian manner with high levels occurring at night. Melatonin has been implicated in photoperiod regulation of various neural and endocrine processes, including seasonal reproduction, body weight, metabolism, and coat color (Masana and Dubocovich, 2001). In mammals, the melatonin rhythm is generated by an endogenous circadian clock in the suprachiasmatic nucleus (SCN) of the hypothalamus, which is entrained by the light/dark cycle to the 24h day. Melatonin may be effective in synchronizing disturbed circadian rhythms in humans, and there is considerable interest in melatonin's potential for treatment of disordered circadian rhythms in jet-lag, shift work, blindness and in delayed/advanced sleep phase syndromes (Hagan and Oakley, 1995). The two mammalian melatonin receptor subtypes, MT1 and MT2, are G-protein coupled receptors which signal through inhibition of cyclic AMP synthesis (Dubocovich et al., 2000). In addition, MT1 is involved in regulation of proliferation of human breast cancer cells (Ram et al., 2002; Collins et al., 2003). Millipore's MT1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of MT1 interactions with its ligands. The membrane preparations exhibit a Kd of 1.7 nM for [125I]-Iodomelatonin. With 0.5 nM [125I]-Iodomelatonin, 5 µg/well MT1 Membrane Prep typically yields greater than 15-fold signal-to-background ratio. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human MT1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_005958.3
Entrez基因汇总 This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. 基因符号 - MEL-1A-R
- MTNR1A
- MT1
- Mel-1A-R
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 MT1 靶子系列 Melatonin UniProt编号 - P48039
UniProt汇总 FUNCTION: SwissProt: P48039 # High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
SIZE: 350 amino acids; 39375 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in hypophyseal pars tuberalis and hypothalamic suprachiasmatic nuclei (SCN). Hippocampus.
SIMILARITY: SwissProt: P48039 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 500mM NaCl. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, filtered and stored at 4°C
Radioligand: [125I] Iodomelatonin (Perkin Elmer#: NEX-236)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 15-fold signal:background with 125I-labeled Iodomelatonin at 0.5 nM产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with MT1 membrane prep and unlabeled melatonin:
10 µg/well Signal:Background 28.5 Specific Binding (cpm) 104301
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [125I]-Iodomelatonin binding: 22.8 pmol/mg protein
Kd for [125I]-Iodomelatonin binding: ~1.7 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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