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merck millipore,默克密理博,MAB5292,Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B
产品名称：Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B
产品型号：MAB5292
This Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B is validated for use in IH, WB for the detection of Dopamine D1B Receptor.
merck millipore,默克密理博,MAB5292,Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5292 Replaces AB1791P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B Alternate Names - Dopamine D5 Receptor
产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 This Anti-Dopamine D1B Receptor Antibody, clone SG4-D1B is validated for use in IH, WB for the detection of Dopamine D1B Receptor. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blot: 1:250-1:500 on mouse brain lysate (unboiled sample)
Immunohistochemistry
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
Solutions
10X Transfer buffer 1 liter
Tris 115.3 gm
Glycine 24.2 gm
[SDS 4 gm (may be omitted)]
For a 1X working solution: 1 liter
mix 700 mL water
200 mL
MeOH100 mL
10X Transfer
TBSTstock 1 liter1M
Tris pH 7.5 10 mL
5 M NaCl 30 mL
10% Tween-20 5 Ml
1. Run SDS-PAGE gel as desired.
2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.
3. Soak membrane in transfer buffer (see below) until ready to set up blot (>15 min) transfer.
4. Assemble gel-transfer sandwich with the blot toward the positive electrode.
5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.
6. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
7. Destain with deionized water.
8. Block with 5% non-fat milk (Marvel or Carnation) in water, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
9. Incubation with first antibody overnight with rocking at 4°C in blocking solution. Optimal working dilutions and incubation time will need to be determined by the end user.
10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.
11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.
12. Wash at least 3 x 5 min. with TBST.
13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).生物信息 免疫原品种 Recombinant rat D1b Receptor, carboxy terminus. 克隆 SG4-D1B 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Dopamine Receptor D1b (D5). 同种型 IgG1 品种反应性 RatMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000798.3
Entrez基因汇总 This gene encodes the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2. 基因符号 - MGC10601
- DRD5
- DRD1B
- DRD1L2
- DBDR
- D
非反应性品种 - Human
UniProt编号 - P21918
UniProt汇总 FUNCTION: SwissProt: P21918 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 477 amino acids; 52951 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Neuron-specific, localized primarily within limbic regions of the brain.
DISEASE: SwissProt: P21918 # Defects in DRD5 may be a cause of schizophrenia, but no proof has yet been found. & Defects in DRD5 are a cause of blepharospasm [MIM:606798]. It is a primary focal dystonia affecting the orbicularis oculi muscles, usually beginning in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. In severe cases, this can lead to functional blindness.
SIMILARITY: SwissProt: P21918 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,20-163,SDS Lysis Buffer - for use in ChIP Assay
下一件merck millipore产品：merck millipore,默克密理博,AB1536,Anti-Dopamine β Hydroxylase Antibody, CT
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