- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MAB5262,Anti-Neurofilament 200 kDa Antibody, clone RT97
产品名称：Anti-Neurofilament 200 kDa Antibody, clone RT97
产品型号：MAB5262
Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB.
merck millipore,默克密理博,MAB5262,Anti-Neurofilament 200 kDa Antibody, clone RT97
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5262 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament 200 kDa Antibody, clone RT97 背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section. 产品信息 格式 Purified 控制 - Cultured neurons
- Brain
演示 Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. 应用 应用 Anti-Neurofilament 200 kDa Antibody, clone RT97 is an antibody against Neurofilament 200 kDa for use in IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
1-10 μg/mL
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human Cerebellum
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Rat brain protein preparation 克隆 RT97 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type). 同种型 IgG1 品种反应性 RatHumanMouse Species Reactivity Note Human and mouse. Expected to react with rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_021076.2
基因符号 - KIAA0845
- NEFH
- NFH
- NF-H
纯化方法 Protein A purfied UniProt编号 - P12036
UniProt汇总 FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
SIZE: 1026 amino acids; 112480 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.产品使用声明 质量保证 Evaluated by Western Blot on Mouse brain membrane lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,171609,Anti-β-Amyloid₄₂ (FCA3542) Rabbit pAb
下一件merck millipore产品：merck millipore,默克密理博,567020,Simvastatin - CAS 79902-63-9 - Calbiochem
相关产品
merck millipore,默克密理博,MABC776,Anti-TCTP, clone EPR5540, Rabbit Monoclonal Antibody
merck millipore,默克密理博,ABE250,Anti-dimethyl Histone H3 (Lys4) Antibody
merck millipore,默克密理博,171609,Anti-β-Amyloid₄₂ (FCA3542) Rabbit pAb
merck millipore,默克密理博,567020,Simvastatin - CAS 79902-63-9 - Calbiochem
merck millipore,默克密理博,171538,AMPK (α1/β1/γ2), His•Tag, Human, Recombinant, S. frugiperda
merck millipore,默克密理博,SF-1122,NPM1; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,208880,Anti-Calnexin, C-Terminal (575-593) Rabbit pAb
merck millipore,默克密理博,GF439,HumanKine TGF beta 1, Human Recombinant Xeno-Free