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merck millipore,默克密理博,ABE250,Anti-dimethyl Histone H3 (Lys4) Antibody
产品名称：Anti-dimethyl Histone H3 (Lys4) Antibody
产品型号：ABE250
Anti-dimethyl Histone H3 (Lys4) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 dimethylated at lysine 4. This purified polyclonal antibody, also known as H3K4me2, has dot blot (D More>>
merck millipore,默克密理博,ABE250,Anti-dimethyl Histone H3 (Lys4) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ch, H, M, R ChIP, WB, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE250 Replaces 04-791 描述 Anti-dimethyl Histone H3 (Lys4) Antibody Alternate Names - histone cluster 3, H3, H3K4me2
- histone 3, H3
- H3 histone family, member T
背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Dimethylation of histone H3 at Lys4 correlates with transcriptional activity of many genes. 产品信息 格式 Affinity Purified 控制 - HeLa acid extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-dimethyl Histone H3 (Lys4) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 dimethylated at lysine 4. This purified polyclonal antibody, also known as H3K4me2, has dot blot (DB) proven specificity & has been validated in WB, ICC, ChIP. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 and A431 cells.
Dot Blot Analysis:
1:1,000 dilution from a representative lot specifically detected Histone H3 in a Lys4 dimethhyl modified histone H3 peptide while not detecting potential cross-reacting peptides corresponding to other modified and non-modified histones.
Chromatin Immunoprecipitation Analysis:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal IgG (Part No. 12-370), or 4 µL of Anti-Dimethyl Histone H3 (Lys4) (Part No. ABE250) and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of Dimethyl-Histone H3 (Lys4) associated DNA fragments was verified by qPCR using primers specific for the human GAPDH coding region.
Please refer to the EZ-Magna ChIP™ A/G (Cat. # 17-10085) protocol for experimental details.
ChIP-Sequencing
Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 5 µg ofa representative lot of anti-dimethyl-Histone H3 (Lys4) antibody(ABE250), 20 µL Protein A/G beads ,and 5e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq™ instrument. An excess of twelve million reads from FastQ files were mapped using Bowtie following TagDust tag removal. Peaks were identified using MACS , with peaks and reads visualized as a custom track in UCSC Genome Browser from BigWig and BED files.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding Histone H3 methylated at Lys4. 表位 Dimethylated Lys4 宿主 Rabbit 特异性 This antibody recognizes Histone H3 dimethylated at Lys4. 品种反应性 ChickenHumanMouseRat Species Reactivity Note Demonstrated to react with human, mouse, rat, and chicken. Broad species cross-reactivity expected based on sequence similarity. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003484.1
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]. 基因符号 - HIST1H3J
- H3FJ
- HIST3H3
- H3FT
- H3T
- MGC126886
- HIST1H3A
- H3FA
- HIST1H3B
- H3FL
- HIST1H3C
- H3FC
- HIST1H3D
- H3FB
- HIST1H3E
- H3FD
- HIST1H3F
- H3FI
- HIST1H3G
- H3FH
- HIST1H3H
- H3FK
- HIST1H3I
- H3FF
修改 - Methylation
纯化方法 Affinity purified UniProt编号 - Q16695
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: Belongs to the histone H3 family.产品使用声明 质量保证 Evaluated by Western Blot using HeLa acid extract and recombinant histone proteins.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H3 on 10 µg of HeLa acid extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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