merck millipore,默克密理博,MAB5256A5,Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H, R, M, Po	IHC, ICC	M	AlexaFluor®555	Monoclonal Antibody

描述
产品目录编号	MAB5256A5
描述	Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor® 555 Conjugate
Alternate Names	Neurofilament triplet H protein Neurofilament heavy polypeptide NF-H 200 kDa neurofilament protein
背景信息	Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

产品信息
格式	AlexaFluor®555
控制	Rat E18 primary cortex cells
演示	Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

应用
应用	Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.
主要应用	Immunohistochemistry Immunocytochemistry
应用说明	Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurofilament in adult mouse brain cryosections.

生物信息
免疫原品种	Purified neurofilament polypeptides (Debus et al., 1983).
克隆	NE14
宿主	Mouse
同种型	IgG1
品种反应性	HumanRatMousePig
Species Reactivity Note	Demonstrated to react with Mouse, and Rat. Predicted to react with Porcine based on immunogen design. Predicted to react with Human based on 100% sequence homology. Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_066554
基因符号	NFH NEFH
纯化方法	Protein A purified
UniProt编号	P12036

产品使用声明
质量保证	Evaluated by Immunocytochemistry in rat E18 primary cortex cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,MAB5256A5,Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate

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