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品种反应性 主要应用 宿主 格式 抗体类型 H ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB4617 Replaces MAB3509 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Survivin Antibody, clone 8E2 Alternate Names - TIAP
背景信息 Survivin, an inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It indicates that survivin may counteract a default induction of apoptosis in G2/M phase. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis. 产品信息 格式 Purified 演示 Purified immunoglobulin, by Protein G. Liquid in 10 mM PBS, pH 7,4 containing 0.2% BSA and 0.09% sodium azide. 应用 应用 Detect Survivin using this Anti-Survivin Antibody, clone 8E2 validated for use in ELISA & IF. 主要应用 - ELISA
不建议的应用 - Western Blotting
应用说明 Immunofluorescence
ELISA (for detection only)
Does not work for Western Blot
Does not work for Immunohistochemistry
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Full length recombinant human survivin 克隆 8E2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 MAB4617 detects human survivin (16.5 kDa). 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001168.2
- NM_001012270.1
- NM_001012271.1
Entrez基因汇总 This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. 基因符号 - API4
- BIRC5
- SVV
- IAP4
- survivin
- EPR-1
UniProt编号 - O15392
UniProt汇总 FUNCTION: SwissProt: O15392 # May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Interacts with tubulin. Inhibitor of caspase-3 and caspase-7. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Isoforms 2 and 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti- apoptotic effects when compared with the displayed wild-type isoform.
SIZE: 142 amino acids; 16390 Da
SUBUNIT: Homodimer. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-caspase-9, as well as active caspase- 9, but much less efficiently. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with EVI5.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
TISSUE SPECIFICITY: Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
DOMAIN: SwissProt: O15392 The BIR repeat is necessary and sufficient for HBXIP binding.
SIMILARITY: Belongs to the IAP family. & Contains 1 BIR repeat.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 12 months. 包装信息 数量 50 µg
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