merck millipore,默克密理愽
  • merck millipore,默克密理博,17-495,PIP3 Quantification TR-FRET Assay

  • merck millipore,默克密理博,17-495,PIP3 Quantification TR-FRET Assay

    产品名称：PIP3 Quantification TR-FRET Assay
    产品型号：17-495
    The PIP3 Quantification TR-FRET Assay kit provides an easy to use method for assaying PIP3 levels by detecting the displacement of biotin-PIP3 from an energy transfer complex.

    merck millipore,默克密理博,17-495,PIP3 Quantification TR-FRET Assay

  • 产品介绍
  • merck millipore,默克密理博,17-495,PIP3 Quantification TR-FRET Assay

    重要规格表

    主要应用 可用的分析物 检测方法
    KAPI 3-KinaseHTRF, TR-Fret
    描述
    产品目录编号 17-495
    描述 PIP3 Quantification TR-FRET Assay
    概述 PI 3-Kinases α, β, γ and δ (h) catalyse the phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg2+. The PIP3 product is detected by displacement of biotin-PIP3 from an energy transfer complex consisting of Europium labelled anti-GST monoclonal antibody, a GSTtagged pleckstrin homology (PH) domain, biotinylated PIP3 and Streptavidin-Allophycocyanin (APC). Excitation of Europium in the complex results in an energy transfer to the APC and a fluorescent emission at 665 nm. The PIP3 product isolated from cell membranes displaces biotin-PIP3 from the complex resulting in a loss of energy transfer and thus a decrease in signal.
    产品信息
    部件
    • 1M DTT
    • Black 96-well plate
    • Detection Buffer
    • DM B
    • DM A
    • Stop B
    • PIP3 Standard
    • Lipid Recovery Buffer
    检测方法 HTRFTR-Fret
    应用
    应用 The PIP3 Quantification TR-FRET Assay kit provides an easy to use method for assaying PIP3 levels by detecting the displacement of biotin-PIP3 from an energy transfer complex.
    主要应用
    • Kinase Assay
    应用说明 This assay requires lipid extraction from cells. A protocol is included with this assay.
    生物信息
    可用的分析物
    • PI 3-Kinase
    Entrez基因编号
    • NM_006218.2
    Entrez基因汇总 Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
    基因符号
    • PIK3CA
    • p110-alpha
    • MGC142161
    • PI3K
    • MGC142163
    UniProt编号
    • P42336
    UniProt汇总 FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
    SIZE: 1068 amino acids; 124284 Da
    SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
    DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
    SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 plate
    包装 Kit capacity: 96-wells

    merck millipore,默克密理博,17-495,PIP3 Quantification TR-FRET Assay

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