merck millipore,默克密理博,MAB4354,Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Pm, H, Mk, Bab, Chp	FC	M	Alkaline Phosphatase	Monoclonal Antibody

描述
产品目录编号	MAB4354
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J
背景信息	In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification. Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

产品信息
格式	Alkaline Phosphatase
控制	Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
演示	Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl pH 7.6 with 0.1% Sodium Azide

应用
应用	Detect TRA-2-54 using this Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J validated for use in FC, IF & IP.
主要应用	Flow Cytometry
应用说明	This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells. FACS Analysis: a starting range of 5-20 μg/ml is suggested. Immunoprecipitation Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	2102Ep human Embryonal Carcinoma Cells
表位	Liver/Bone/Kidney Alkaline Phosphatase
克隆	TRA-2-54/2J
宿主	Mouse
特异性	Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody does not cross react with other isozymes of AP.
同种型	IgG1
品种反应性	PrimateHumanMonkeyBaboonChimpanzee
Species Reactivity Note	Reactivity has been demonstrated with Liver / Bone / Kidney isozyme of Alkaline Phospatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon) and Old World Monkeys (Baboon, Rhesus Monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey). No reactivity has been observed with New World Monkeys (Owl Monkey, Squirrel Monkey), Bovine, Canine, Cat, Goat, Guinea Pig, Hamster, Mouse, Pig, Rabbit, Rat Sheep and Tiger.
抗体类型	Monoclonal Antibody
基因符号	TRA-3-54
非反应性品种	Feline Guinea Pig Mouse Pig Rat Rabbit

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MAB4354,Anti-TRA-2-54 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-54/2J

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