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merck millipore,默克密理博,MAB4314,Anti-CD93 Antibody, extracellular, clone R139
产品名称：Anti-CD93 Antibody, extracellular, clone R139
产品型号：MAB4314
This Anti-CD93 Antibody, extracellular, clone R139 is validated for use in FC, IP, WB for the detection of CD93.
merck millipore,默克密理博,MAB4314,Anti-CD93 Antibody, extracellular, clone R139
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4314 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CD93 Antibody, extracellular, clone R139 Alternate Names - Complement C1q Receptor
产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in 0.02 M Phosphate with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative. 应用 应用 This Anti-CD93 Antibody, extracellular, clone R139 is validated for use in FC, IP, WB for the detection of CD93. 主要应用 - Western Blotting
- Flow Cytometry
应用说明 Immunocytochemistry:
Recommended antibody dilution: 5-10 μg/ml
Fixative: 4% Paraformaldehyde / PBS
Blocking buffer: 2% BSA / 2% Normal serum / PBS
Dilution Buffer: 2% BSA / 2% Normal serum / PBS
Incubation Times/Temperature: 1 hour at room temperature
Recommended control tissue: Positive: U937 monocytes; Negative: T cells
Immunoblotting:
Recommended primary antibody dilution: 5 μg/ml
Dilution buffer: 3% milk powder / 0.05%Tween 20 in Tris buffered saline
Sample preparation: Denatured only (SDS & boiled)
Preferred Gel percentage: 7.5% Polyacrylamide
Blocking Buffer: 3% milk powder / 0.05% Tween 20 in Tris buffered saline
Incubation time: 2 hours at room temperature
Expected size of band: 97 kDa
Recommended control extracts: Positive: U937 monocytes; Negative: T cells
Immunoprecipitation:
Recommended primary antibody dilution: 5 μg/ml
Cell lysis buffer: 10mM TEA, 1mM Calcium Cholride, 0.15M Sodium Chloride, 0.3% NP40 and protease inhibitors
Final Reaction volume: 1000 μl
Suggested total protein concentration: 0.15 mg/ml (1 x 107 cells)
Incubation time: 1 hour at 4°C
Expected size of band: 97kDa
ELISA (Indirect):
Recommended coating concentration: 10 μg/ml
Dilution buffer: 0.1M Sodium Carbonate pH9.4
FACS Analysis:
Suggested dilution/number of cells: 2 μg/million cells
Fixation/Permeabilization used: 1% Paraformaldehyde / PBS
Recommended controls: U937 Monocytes
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 C1q collagen like domain binding proteins 表位 extracellular 克隆 R139 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes human CD93 (C1qRp), a 90-120kD protein that is highly expressed on monocytes/macrophages, neutrophils and granulocytes. CD93 is not expressed upon T and B lymphocytes. C1qRp binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). However, CD93 is not necessary for the binding of C1q or for inducing C1q-mediated enhancement of phagocytic activity.Recent evidence suggests that CD93 may define a new human stem cell population with hematopoietic and hepatic potential3. 同种型 IgG2b 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_012072.3
Entrez基因汇总 The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. 基因符号 - C1qRP
- CD93
- MXRA4
- C1qRp
- C1qR(P)
- C1qR
- dJ737E23.1
- C1QR1
- CDw93
- p
UniProt编号 - Q9NPY3
UniProt汇总 FUNCTION: SwissProt: Q9NPY3 # Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.
SIZE: 652 amino acids; 68560 Da
SUBUNIT: Interacts with HCV core protein.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.
PTM: N- and O-glycosylated.
SIMILARITY: SwissProt: Q9NPY3 ## Contains 1 C-type lectin domain. & Contains 5 EGF-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 100 µg
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