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merck millipore,默克密理博,MAB4190,Anti-Ki-67 Antibody, clone Ki-S5
产品名称：Anti-Ki-67 Antibody, clone Ki-S5
产品型号：MAB4190
Anti-Ki-67 Antibody, clone Ki-S5 is a high quality Mouse Monoclonal Antibody for the detection of Ki-67 & has been validated in FC, ICC, IHC, IHC(P) & WB.
merck millipore,默克密理博,MAB4190,Anti-Ki-67 Antibody, clone Ki-S5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, FC, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4190 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Ki-67 Antibody, clone Ki-S5 背景信息 Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer. 产品信息 格式 Purified 控制 - Tonsil tissue, A431 cell lysate
演示 Mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 Anti-Ki-67 Antibody, clone Ki-S5 is a high quality Mouse Monoclonal Antibody for the detection of Ki-67 & has been validated in FC, ICC, IHC, IHC(P) & WB. 主要应用 - Western Blotting
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry:
A 5-10 μg/mL concentration of a previous lot was used in IC.
Immunohistochemistry:
A 5-10 μg/mL concentration of a previous lot was used in IH.
Immunohistochemistry:
5-10 µg/mL on formalin fixed paraffin tissue. Citrate buffer-microwave antigen retrieval
Flow Cytometry:
A previous lot of this antibody was used in FC.
Western blot:
1-10 µg/mL
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Nuclear protein preparation from the human cell line U937.50. 克隆 Ki-S5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 In Immunoprecipitation and Western blot experiments the Ki-S5 antibody recognizes a cell-cycle-associated protein of 345 kD and 395 kD (Kreipe et al., 1993) identical with the Ki-67 antigen. The antibody binds to a formalin-resistant epitope of the Ki-67 antigen. The immunoreactivity of Ki-S5 is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval (Mauri et al., 1984). A comparison of immunohistochemical labeling of fresh and fixed tissue samples of NHL showed that identical results were obtained with Ki-67 and Ki-S5 (Kreipe et al., 1993). The Ki-67 antigen is preferentially expressed during late G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack the Ki-67 antigen. In addition, constantly proliferating cells (e.g. cell lines) react positively to Ki-S5 during the entire cell-cycle. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer. 同种型 IgG1 品种反应性 Human Species Reactivity Note Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002417.3
基因符号 - Ki-67
- MKI67
- KIA
纯化方法 Protein A purfied UniProt编号 - P46013
UniProt汇总 FUNCTION: SwissProt: P46013 # Thought to be required for maintaining cell proliferation.
SIZE: 3256 amino acids; 358694 Da
SUBUNIT: Binds through the FHA domain to MKI67IP.
SUBCELLULAR LOCATION: Nucleus. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
DEVELOPMENTAL STAGE: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
SIMILARITY: SwissProt: P46013 ## Contains 1 FHA domain.产品使用声明 质量保证 Evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected cell-cycle-associated protein of 345 kDa and 395 kDa on 10 μg of A431 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,PF033,Fas Ligand, Human, Recombinant
下一件merck millipore产品：merck millipore,默克密理博,AB3066,Anti-Aquaporin 2 Antibody
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