- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3066 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Aquaporin 2 Antibody Alternate Names - MCH-CD
- AQP2
- AQP-CD
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin.Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide. 应用 应用 Detect Aquaporin 2 using this Anti-Aquaporin 2 Antibody validated for use in ELISA, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Western Blotting: 1-10 μg/mL for affinity pure antibody using Chemiluminescence technique. An antibody made to the AQP2 peptide detected a major band at ~29 kDa in rat kidney cortex, and 35-47 kDa in inner and outer medulla (postranslational or glycosylation) (Nielsen et al. 1993; Deen et al. 1995). A similar pattern is seen in the human kidney (Sasaki, et al. 1994).
Immunohistochemistry: 2-10 μg/mL in paraformaldehyde fixed sections of tissues. AQP2 has been localized in apical membranes (Fushimi et al. 1993; Nielsen et al. 1993; Deen et al. 1995).
ELISA: 0.5-1.0 μg/mL using 1 μg/mL control peptide/well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A 15 aa C-terminal peptide from rat AQP2. This peptide is predicted to be cytoplasmic. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Aquaporin 2 (AQP-CD/WCH-CD).
SPECIES REACTIVITY: The antibody has not yet been tested for cross-reactivity to other species. The 15 aa C-terminal peptide has 100% homology with mouse and 93% homology with human AQP2.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000486.3
Entrez基因汇总 This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus. 基因符号 - WCH-CD
- AQP2
- AQP-CD
- aquaporin-CD
- Aquaporin-2
- Aquaporin-CD
- AQP-2
- MGC34501
UniProt编号 - P41181
UniProt汇总 FUNCTION: SwissProt: P41181 # Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
SIZE: 271 amino acids; 28837 Da
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane.
TISSUE SPECIFICITY: Expressed in renal collecting tubules.
DOMAIN: SwissProt: P41181 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
PTM: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
DISEASE:SwissProt: P41181 # Defects in AQP2 are a cause of autosomal nephrogenic diabetes insipidus, type I (AD-NDI) [MIM:125800]. It is characterized by excessive water drinking (polydypsia) and urine excretion (polyuria).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB4190,Anti-Ki-67 Antibody, clone Ki-S5
下一件merck millipore产品：merck millipore,默克密理博,MABD131,Anti-ISL1 Antibody, clone 1H9
相关产品
merck millipore,默克密理博,559304,Anti-SAPK/JNK Rabbit pAb
merck millipore,默克密理博,SVHV01015,Sterivex-HV 过滤器，0.45 µm，PVDF，射线灭菌
merck millipore,默克密理博,401799,Human IgM, Plasma
merck millipore,默克密理博,PF033,Fas Ligand, Human, Recombinant
merck millipore,默克密理博,MAB4190,Anti-Ki-67 Antibody, clone Ki-S5
merck millipore,默克密理博,MABD131,Anti-ISL1 Antibody, clone 1H9
merck millipore,默克密理博,07-344,Anti-CPI-17 Antibody
merck millipore,默克密理博,AB15184P,Anti-Fbx2 Antibody
merck millipore,默克密理博,21-170,TOPflash (TCF Reporter Plasmid)