merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB4092,Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

  • merck millipore,默克密理博,MAB4092,Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

    产品名称：Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1
    产品型号：MAB4092
    Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

    merck millipore,默克密理博,MAB4092,Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

  • 产品介绍
  • merck millipore,默克密理博,MAB4092,Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWB, FC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB4092
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1
    Alternate Names
    • CD162
    • PSGL-1
    背景信息 Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.
    产品信息
    格式 Purified
    演示 Purified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.
    应用
    应用 Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunohistochemistry
    应用说明 Western blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000

    FACS {Snapp, KR et al 1998}.

    Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).

    Functional blocking of P-selectin {Snapp KR et al 1998}.

    Immunohistochemistry: Fresh frozen, acetone fixed sections

    Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
    克隆 KPL-1
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Known not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_003006.3
    Entrez基因汇总 SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
    基因符号
    • PSGL1
    • SELPLG
    • CLA
    • PSGL-1
    • CD162
    UniProt编号
    • Q14242
    UniProt汇总 FUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells.
    SIZE: 412 amino acids; 43201 Da
    SUBUNIT: Homodimer; disulfide-linked.
    SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
    TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes.
    PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C in undiluted aliquots up to 12 months.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB4092,Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

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