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品种反应性 主要应用 宿主 格式 抗体类型 Mk, H IHC, ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB3826 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Mucin 5B Antibody, clone 19.4E Alternate Names - MUC5B
背景信息 Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 103 kDa. 产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide. 应用 应用 Detect Mucin 5B using this Anti-Mucin 5B Antibody, clone 19.4E validated for use in ELISA, WB, IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Western blot; Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins.
Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).
ELISA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Un-glycosylated, serine- and threonine-free synthetic peptide from the N-terminus of human MUC5B. 克隆 19.4E 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C. 同种型 IgG1 品种反应性 MonkeyHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002458.1
基因符号 - MUC9
- MUC5B
- MG1
- MUC5
UniProt编号 - Q9HC84
UniProt汇总 FUNCTION: SwissProt: Q9HC84 # Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
SIZE: 5703 amino acids; 590499 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
DOMAIN:SwissProt: Q9HC84 The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.
PTM: Highly glycosylated. C-, N- and O-gylcosylated. C- mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr- rich tandem repeat (TR) region. The repeat region is about 59% O- glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
SIMILARITY: Contains 1 CTCK (C-terminal cystine knot-like) domain. & Contains 1 TIL (trypsin inhibitory-like) domain. & Contains 3 VWFC domains. & Contains 4 VWFD domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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