merck millipore,默克密理博,MAB378,Anti-MAP2A Antibody, 2B

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merck millipore,默克密理博,MAB378,Anti-MAP2A Antibody, 2B
产品名称：Anti-MAP2A Antibody, 2B
产品型号：MAB378
Detect MAP2A using this Anti-MAP2A Antibody, 2B validated for use in IH & WB.

merck millipore,默克密理博,MAB378,Anti-MAP2A Antibody, 2B

产品介绍

merck millipore,默克密理博,MAB378,Anti-MAP2A Antibody, 2B

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Qu, Am, Av, B, H, M, R, Xn	WB, IHC	M	Ascites	Monoclonal Antibody

描述
产品目录编号	MAB378
Replaces	MAB3418
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-MAP2A Antibody, 2B
Alternate Names	Microtubule Associated Protein 2
背景信息	MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984). MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).

产品信息
格式	Ascites
控制	Cultured neurons Brain tissue
演示	Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide

应用
应用	Detect MAP2A using this Anti-MAP2A Antibody, 2B validated for use in IH & WB.
主要应用	Western Blotting Immunohistochemistry
应用说明	Immunohistochemistry: 1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry. Western blot: SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Western blot: A 1:500 dilution was used in a previous lot. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Bovine MAP2
克隆	AP20
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	MAP2a & b. Does not recognize other variants.
同种型	IgG1
品种反应性	QuailAmphibianAvianBovineHumanMouseRatXenopus
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001039538.1
Entrez基因汇总	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
基因符号	MAP2A MAP2 MAP2B MAP-2 MAP2C DKFZp686I2148
纯化方法	Unpurified
UniProt编号	P11137
UniProt汇总	FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.

产品使用声明
质量保证	Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.