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品种反应性 主要应用 宿主 格式 抗体类型 M WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1498P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Stem Cell Factor Antibody Alternate Names - MGF
- SCF
- c-kit Ligand
- Mast Cell Growth Factor
背景信息 Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble form is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors. 产品信息 格式 Affinity Purified 控制 - Human mast cells
演示 Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water. 应用 应用 Detect Stem Cell Factor using this Anti-Stem Cell Factor Antibody validated for use in ELISA, WB, NEUT. 主要应用 - Western Blotting
- ELISA
应用说明 ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at lease 0.5 μg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant mSCF.
Neutralization: For ½ maximal inhibition (ND50) of the bioogical activity of mSCF (20 ng/mL), a concentration of 0.64 - 0.97 μg/mL is required.
Western blot: 0.1 - 0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Highly pure (>98%) recombinant mouse SCF 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Reacts with mouse SCF; SCF is highly conserved cross reactivity with rat, human and other species SCF is expected but untested. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003994.4
- NM_000899.3
Entrez基因汇总 This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. 基因符号 - KL-1
- KITLG
- SCF
- MGF
- Kitl
- DKFZp686F2250
- SF
UniProt编号 - P21583
UniProt汇总 FUNCTION: SwissProt: P21583 # Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins.
SIZE: 273 amino acids; 30899 Da
SUBUNIT: Homodimer, non-covalently linked (Probable).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Also exists as a secreted soluble form (isoform 1 only) (By similarity). & Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity).
DEVELOPMENTAL STAGE: Acts in the early stages of hematopoiesis.
PTM: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
SIMILARITY: SwissProt: P21583 ## Belongs to the SCF family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile water to a concentration of = 0.2 mg/ml the antibody is stable for at least six weeks at 2-4ºC. 包装信息 数量 50 µg
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