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merck millipore,默克密理博,MAB365,Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
产品名称：Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
产品型号：MAB365
Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB.
merck millipore,默克密理博,MAB365,Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
- 产品介绍
- merck millipore,默克密理博,MAB365,Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB365 Replaces 04-1111 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG Alternate Names - p75 ICD
- CD271 antigen
- Low affinity neurotrophin receptor p75NTR
- Low-affinity nerve growth factor receptor
- NGF receptor
- low affinity nerve growth factor receptor
- nerve growth factor receptor
- nerve growth factor receptor (TNFR superfamily, member 16)
- member 16)
背景信息 The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells. 产品信息 格式 Purified 控制 - Western Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.
演示 Mouse monoclonal IgG1 in 0.02M Phosphate buffer, pH7.6, 0.25M NaCl with 0.1% sodium azide 应用 应用 Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western Blot:
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.
Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.
Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.
Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.生物信息 免疫原品种 n-octoglucoside stabilized proteins containing p75 receptor, which were isolated from plasma membranes of PC-12 cells (Chandler et al., 1984). 表位 Extracellular 克隆 192-IgG 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Specific for the rat low affinity Nerve Growth Factor-Receptor (p75), does not react with human or mouse p75. 同种型 IgG1 品种反应性 Rat Species Reactivity Note Reacts with rat p75 protein. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002507.2
Entrez基因汇总 Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. 基因符号 - CD271
- NGFR
- p75(NTR)
- TNFRSF16
- Gp80-LNGFR
纯化方法 Protein A purfied UniProt编号 - P08138
UniProt汇总 FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
SIZE: 427 amino acids; 45183 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.产品使用声明 质量保证 Immunohistochemistry:
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 50 µg
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