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merck millipore,默克密理博,MAB3580,Anti-Green Fluorescent Protein Antibody
产品名称：Anti-Green Fluorescent Protein Antibody
产品型号：MAB3580
Detect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.
merck millipore,默克密理博,MAB3580,Anti-Green Fluorescent Protein Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A WB, ELISA, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB3580 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Green Fluorescent Protein Antibody Alternate Names - eGFP
- GFP
背景信息 The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. 产品信息 格式 Purified 控制 - GFP fusion protein expressed in cells
演示 Liquid in PBS containing 1 mg/mL BSA. Contains no preservative. 应用 应用 Detect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.
ELISA: 1:1,000-1:2,000 in a direct assay.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GFP-BSA 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry. 同种型 IgG1 品种反应性 All 抗体类型 Monoclonal Antibody 纯化方法 Protein A purfied UniProt编号 - P42212
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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