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merck millipore,默克密理博,MAB3472,Anti-Hypoxia Inducible Factor 2 α Antibody, clone 190b
产品名称：Anti-Hypoxia Inducible Factor 2 α Antibody, clone 190b
产品型号：MAB3472
Use Anti-Hypoxia Inducible Factor 2 α Antibody, clone 190b (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect Hypoxia Inducible Factor 2 α also known as HIF-2 alpha.
merck millipore,默克密理博,MAB3472,Anti-Hypoxia Inducible Factor 2 α Antibody, clone 190b
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 MAB3472 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Hypoxia Inducible Factor 2 α Antibody, clone 190b Alternate Names - HIF-2 alpha
产品信息 格式 Ascites 演示 Ascites fluid. Liquid. Contains no preservative. 应用 应用 Use Anti-Hypoxia Inducible Factor 2 α Antibody, clone 190b (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect Hypoxia Inducible Factor 2 α also known as HIF-2 alpha. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western blot on whole cell extracts. Suggested blocking solution is PBS containing 5% non-fat dried milk and 0.1% Tween 20.
Immunohistochemistry on paraffin embedded tissue sections. Recommended antigen retrieval: pressure cooking in 50 mmol/L Tris and 0.2 mmol/L EDTA buffer, pH 9.0 for 180 seconds or incubating at 60°C for 16 hours overnight.
Immunocytochemistry on transfected COS-1 cells (snap frozen in liquid nitrogen or fixed overnight in 10% neutral buffered formalin and paraffin embedded).
ELISA
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant human HIF-2 alpha. 克隆 190b 宿主 Mouse 特异性 Hypoxia inducible factor-2 alpha (HIF-2 alpha). 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001430.3
基因符号 - PASD2
- EPAS1
- EPAS-1
- HIF2A
- HLF
- MOP2
UniProt编号 - Q99814
UniProt汇总 FUNCTION: SwissProt: Q99814 # Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
SIZE: 870 amino acids; 96459 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP (By similarity).
SUBCELLULAR LOCATION: Nucleus (Potential).
TISSUE SPECIFICITY: Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
PTM: In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). & In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation (By similarity). & Phosphorylated on multiple sites in the CTAD (By similarity).
SIMILARITY: SwissProt: Q99814 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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