merck millipore,默克密理博,MAB3450,Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6

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merck millipore,默克密理博,MAB3450,Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6
产品名称：Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6
产品型号：MAB3450
Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 gamma also known as HP-1gamma & has been validated in ELISA, ICC More>>

merck millipore,默克密理博,MAB3450,Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6

产品介绍

merck millipore,默克密理博,MAB3450,Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H, R	WB, ELISA, ICC, IHC	M	Ascites	Monoclonal Antibody

描述
产品目录编号	MAB3450
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6
Alternate Names	HP-1gamma
背景信息	Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

产品信息
格式	Ascites
控制	HeLa cell lysate, NIH/3T3, C6 and COS cells.
演示	Unpurified mouse IgG1 monoclonal. Contains no preservative.

应用
应用	Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 gamma also known as HP-1gamma & has been validated in ELISA, ICC, IF, IHC & WB.
主要应用	Western Blotting ELISA Immunocytochemistry Immunohistochemistry
应用说明	Western blot: A 1:500-1:5,000 dilution of a previous lot was used for WB. Immunofluorescence: A 1:500-1:5,000 dilution of a previous lot was used in IF. Immunohistochemistry: A 1:500-1:5,000 dilution of a previous lot was used in IH. ELISA: A 1:500-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Recombinant mouse HP1-gamma
克隆	2MOD-1G6
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes Heterochromatin protein-1 gamma (HP1gamma).
同种型	IgG1
品种反应性	MouseHumanRat
Species Reactivity Note	Human and Mouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_007276.3
Entrez基因汇总	At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
基因符号	HECH CBX3 HP1-GAMMA HP1Hs-gamma
纯化方法	Unpurified
UniProt编号	Q13185
UniProt汇总	FUNCTION: SwissProt: Q13185 # Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. SIZE: 183 amino acids; 20811 Da SUBUNIT: Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A (By similarity). Interacts with MIS12 and C20orf127. Can interact directly with CBX5 via the chromoshadow domain. SUBCELLULAR LOCATION: Nucleus (Potential). Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential). PTM: Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. SIMILARITY: SwissProt: Q13185 ## Contains 2 chromo domains.

产品使用声明
质量保证	Routinely evaluated by Western Blot on A431 lysates. Western Blot Analysis: 1:500 dilution of this lot detected HP-1 GAMMA on 10 μg of A431 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.