merck millipore,默克密理博,06-852,Anti-Spinophilin/Neurabin-II Antibody

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merck millipore,默克密理博,06-852,Anti-Spinophilin/Neurabin-II Antibody
产品名称：Anti-Spinophilin/Neurabin-II Antibody
产品型号：06-852
Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.

merck millipore,默克密理博,06-852,Anti-Spinophilin/Neurabin-II Antibody

产品介绍

merck millipore,默克密理博,06-852,Anti-Spinophilin/Neurabin-II Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R	WB, ICC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-852
品牌系列	Upstate
商名	Upstate
描述	Anti-Spinophilin/Neurabin-II Antibody
Alternate Names	protein phosphatase 1, regulatory subunit 9B, spinophilin Protein phosphatase 1 regulatory subunit 9B 3 neurabin II protein phosphatase 1, regulatory (inhibitor) subunit 9B protein phosphatase 1, regulatory subunit 9B regulatory subunit 9B
背景信息	Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.

产品信息
格式	Purified
控制	Brain tissue
演示	Purified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.

应用
应用	Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation: 4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).

生物信息
免疫原品种	GST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Specific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.
同种型	IgG
品种反应性	Rat
Species Reactivity Note	Rat. Other species not known.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_032595
Entrez基因汇总	Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system (Allen et al., 1997 [PubMed 9275233]).[supplied by OMIM]
基因符号	Neurabin-II PPP1R9B PPP1R6 SPINO Spinophilin PPP1R9 Neurabin-2 FLJ30345 spinophilin
纯化方法	Protein G purfied
UniProt编号	Q96SB3
UniProt汇总	FUNCTION: SwissProt: Q96SB3 # Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha- adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). SIZE: 815 amino acids; 89192 Da SUBUNIT: Possibly exists as an homodimer, homotrimer or an homotetramer. Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D(2) dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse. Note=Enriched at synapse and cadherin- based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). PTM: Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1 (By similarity). SIMILARITY: SwissProt: Q96SB3 ## Contains 1 PDZ (DHR) domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on a rat brain microsomal preparation. Western Blot Analysis: 0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.