merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB3309,Anti-MMP-9 Antibody, near CT, clone 56-2A4

  • merck millipore,默克密理博,MAB3309,Anti-MMP-9 Antibody, near CT, clone 56-2A4

    产品名称：Anti-MMP-9 Antibody, near CT, clone 56-2A4
    产品型号：MAB3309
    Anti-MMP-9 Antibody, near C-terminus, clone 56-2A4 detects level of MMP-9 & has been published & validated for use in ELISA, IH, IH(P) & WB.

    merck millipore,默克密理博,MAB3309,Anti-MMP-9 Antibody, near CT, clone 56-2A4

  • 产品介绍
  • merck millipore,默克密理博,MAB3309,Anti-MMP-9 Antibody, near CT, clone 56-2A4

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, Gp, H, RbWB, ELISA, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB3309
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-MMP-9 Antibody, near CT, clone 56-2A4
    Alternate Names
    • 92 kDa Type IV Collagenase
    • Matrix Metalloproteinase-9
    • Gelatinase B
    背景信息 All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).
    MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.
    产品信息
    格式 Purified
    控制
    • HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
    演示 Purified mouse monoclonal IgG1κ in 0.1 M Phosphate buffer, pH 7.0 containing 2% protease free bovine serum albumin.
    应用
    应用 Anti-MMP-9 Antibody, near C-terminus, clone 56-2A4 detects level of MMP-9 & has been published & validated for use in ELISA, IH, IH(P) & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunohistochemistry
    应用说明 Immunoblotting:
    2-5 µg/mL. Recognizes a band corresponding to pro-MMP-9 at 92kDa and an intermediate form at 83 kDa when evaluated versus the conditioned medium of human fibrosarcoma (HT1080) cells.

    Immunohistochemistry:
    A previous lot was used on frozen or paraffin-embedded tissue sections, PLP Fixation preferred (Nomura, 1996).
    1-5 µg/mL of a previous lot of this antibody will work on formalin fixed tissues (see protocols).

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 A peptide corresponding to amino acids 626-644 of human MMP-9 (RSASEVDRMFPGV PLDTHD).
    表位 Near C-terminus
    克隆 56-2A4
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Antibody MAB3309 is directed against the carboxy terminal domain of human MMP-9, residues 626-644. The antibody specifically reacts with the hMMP-9 and cross-reacts with guinea pig, rat and rabbit MMP-9, but not with the human MMP-1, 2, 3, or 13. The antibody does not react with mouse or bovine MMP-9. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9.
    同种型 IgG1κ
    品种反应性 RatGuinea PigHumanRabbit
    Species Reactivity Note Reacts with Human, Guinea Pig, Rat and Rabbit. Does not react with Mouse, Bovine and Hamster. Reactivity with other species has not been determined.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_004994.2
    Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
    基因符号
    • EC 3.4.24.35 [Contains: 67 kDa matrix metalloproteinase-9 82 kDa matrix metalloproteinase-9].
    • MMP9
    • MMP-9
    • GELB
    • CLG4B
    非反应性品种
    • Hamster
    • Bovine
    • Mouse
    纯化方法 Protein A purfied
    UniProt编号
    • P14780
    UniProt汇总 FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.
    COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.
    SIZE: 707 amino acids; 78427 Da
    SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
    TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
    DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
    MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.
    产品使用声明
    质量保证 Evaluated by Western Blot on Human spleen lysates.

    Western Blotting:
    1:500 dilution of this antibody detected MMP-9 on 10 µg of Human spleen lysates.
    销售限制 This product is not available for sale in Japan.
    使用声明
    • Manufactured by Daiichi Fine Chemical Co., Ltd
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB3309,Anti-MMP-9 Antibody, near CT, clone 56-2A4

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