merck millipore,默克密理博,06-911,Anti-acetyl-Histone H3 (Lys14) Antibody

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merck millipore,默克密理博,06-911,Anti-acetyl-Histone H3 (Lys14) Antibody
产品名称：Anti-acetyl-Histone H3 (Lys14) Antibody
产品型号：06-911
Use Anti-acetyl-Histone H3 (Lys14) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14).

merck millipore,默克密理博,06-911,Anti-acetyl-Histone H3 (Lys14) Antibody

产品介绍

merck millipore,默克密理博,06-911,Anti-acetyl-Histone H3 (Lys14) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Eu, H	WB, ICC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-911
品牌系列	Upstate
商名	Upstate
描述	Anti-acetyl-Histone H3 (Lys14) Antibody
Alternate Names	histone cluster 3, H3 H3K14Ac Histone H3 (acetyl K14) H3 histone family, member T histone 3, H3 histone 3 H3
背景信息	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

产品信息
格式	Purified
控制	Sodium butyrate treated acid extracted proteins from HeLa cells.
演示	Purified rabbit polyclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide and 30% glycerol. Store at -20°C.

应用
应用	Use Anti-acetyl-Histone H3 (Lys14) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14).
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation: 1 μg of a previous lot immunoprecipitated acetylated Histone-H3 from 500 μg of an acid precipitate of sodium butyrate treated HeLa cells. Immunocytochemistry: Not determined.

生物信息
免疫原品种	KLH-conjugated synthetic peptide (ARKSTGG[AcK]APR-GC) corresponding to amino acids 7-17 of Histone-H3.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Histone-H3, Mr 17 kDa, acetylated at position 14.
同种型	IgG
品种反应性	EukaryoteHuman
Species Reactivity Note	Human. This peptide sequence is identical in a wide range of animal and plant species.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_003493
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
基因符号	MGC126886 HIST3H3 H3FT H3/t H3t MGC126888 H3/g H3.4 H3T
修改	Acetylation
纯化方法	Protein A purfied
UniProt编号	Q16695
UniProt汇总	FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.

产品使用声明
质量保证	Routinely evaluated by Western Blot on sodium butyrate treated acid extracted proteins from HeLa cells. Western Blot Analysis: 0.5-1.0 μg/mL of this lot detected acetylated Histone-H3 in acid extracted proteins from HeLa cells. Sodium butyrate, an inhibitor of histone deacetylases, was used to enhance detection of acetylated Histone-H3.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.