merck millipore,默克密理博,MAB3299,Anti-DNA Antibody, single stranded specific, clone F7-26

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
A	IHC, FC, ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB3299
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-DNA Antibody, single stranded specific, clone F7-26
背景信息	Apoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.

产品信息
格式	Purified
控制	Apoptotic cells
演示	Purified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives.

应用
应用	Anti-DNA Antibody, single stranded specific, clone F7-26 detects level of DNA & has been published & validated for use in FC, IC, IH, IH(P).
主要应用	Immunohistochemistry Flow Cytometry Immunocytochemistry
应用说明	1. Immunoprecipitation 2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),

生物信息
免疫原品种	F7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.
表位	single stranded specific
克隆	F7-26
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Specifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.
同种型	IgM
品种反应性	All
抗体类型	Monoclonal Antibody
纯化方法	Ammonium sulfate precipitation

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.

包装信息
数量	50 µg

merck millipore,默克密理博,MAB3299,Anti-DNA Antibody, single stranded specific, clone F7-26

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