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品种反应性 主要应用 宿主 格式 抗体类型 M IHC, WB R Ascites Monoclonal Antibody 描述 产品目录编号 MAB1946 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Nidogen Antibody, clone ELM1 Alternate Names - Entactin
产品信息 格式 Ascites 演示 Ascites. 应用 应用 This Anti-Nidogen Antibody, clone ELM1 is validated for use in DB, IP, WB, IH for the detection of Nidogen. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry: immunofluorescence and immunoperoxidase. For best results use with cryostat sections fixed with acetone or 4% formaldehyde. Has not been tested on paraffin embedded tissue.
Immunoprecipitation
Dot and Western blotting.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Basement membrane protein from mouse EHS tumor. 克隆 ELM1 宿主 Rat 特异性 Reacts with murine entactin/nidogen (MW 146-150 kD) and its large proteolytic fragments. No cross-reactivity to laminin, collagen type IV, perlecan or to fibronectin. In mouse tissues reacts strongly and uniformly with basement membranes. 同种型 IgG2aκ 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002508.2
Entrez基因汇总 This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. 基因符号 - entactin
- NID1
- enactin
- NID
- Entactin
UniProt编号 - P14543
UniProt汇总 FUNCTION: SwissProt: P14543 # Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions.
SIZE: 1247 amino acids; 136453 Da
SUBUNIT: Interacts with FBLN1 and LGALS3BP (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane.
PTM: N- and O-glycosylated.
SIMILARITY: SwissProt: P14543 ## Contains 6 EGF-like domains. & Contains 4 LDL-receptor class B repeats. & Contains 1 NIDO domain. & Contains 1 nidogen G2 beta-barrel domain. & Contains 1 thyroglobulin type-1 domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots up to 12 months. Short term storage (1-6 weeks) maintain at +2-8°C with 0.1% sodium azide. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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