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品种反应性 主要应用 宿主 格式 抗体类型 Rb, B, H, M IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 CBL218 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Keratin K3/K76 Antibody, clone AE5 产品信息 格式 Purified 演示 The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide. 应用 应用 Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P). 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry on frozen sections: 1:50.
Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).
Western blotting: 1:500.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Rabbit corneal epithelial keratin 克隆 AE5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. 同种型 IgG1 品种反应性 RabbitBovineHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_057088.2
Entrez基因汇总 The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmanns Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. 基因符号 - Keratin-3
- KRT3
- CK3
- K3
- CK-3
- Cytokeratin-3
纯化方法 Ion exchange chromatography UniProt编号 - Q01546
- P12035
UniProt汇总 FUNCTION: SwissProt: Q01546 # Probably contributes to terminal cornification.
SIZE: 638 amino acids; 65871 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins.
DEVELOPMENTAL STAGE: Synthesized during maturation of epidermal keratinocytes.
SIMILARITY: SwissProt: Q01546 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,MAB1946,Anti-Nidogen Antibody, clone ELM1
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