merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1922,Anti-Laminin α2 Antibody, clone 5H2

  • merck millipore,默克密理博,MAB1922,Anti-Laminin α2 Antibody, clone 5H2

    产品名称：Anti-Laminin α2 Antibody, clone 5H2
    产品型号：MAB1922
    Detect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB.

    merck millipore,默克密理博,MAB1922,Anti-Laminin α2 Antibody, clone 5H2

  • 产品介绍
  • merck millipore,默克密理博,MAB1922,Anti-Laminin α2 Antibody, clone 5H2

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Rb, H, MkWB, ELISA, CULT, IHCMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB1922
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Laminin α2 Antibody, clone 5H2
    Alternate Names
    • laminin, alpha 2
    • Laminin M chain
    • Merosin heavy chain
    • laminin M
    • laminin alpha 2 subunit
    • laminin
    背景信息 Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis.
    产品信息
    格式 Ascites
    控制
    • Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs
    演示 Unpurified ascites in buffer containing no preservatives.
    应用
    应用 Detect Laminin α2 using this Anti-Laminin α2 Antibody, clone 5H2 validated for use in ELISA, CULT, IF, IH, IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Cell Culture
    • Immunohistochemistry
    应用说明 ELISA:
    50% maximal binding to human merosin at 1:50,000 dilution from a previous lot.

    Immunohistochemistry:
    1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.

    Immunofluorescence:
    A previous lot of this antibody was used in immunofluorescent.

    Affinity chromatography:
    A previous lot of this antibody was used in IAP.

    Immunoprecipitation:
    A previous lot of this antibody was used in IP.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Purified human merosin
    克隆 5H2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reacts with the 80 kDa fragment of the M-chain of human merosin.
    同种型 IgG1
    品种反应性 RabbitHumanMonkey
    Species Reactivity Note Cross reacts with monkey and rabbit merosin.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_000426.3
    Entrez基因汇总 Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.
    基因符号
    • LAMM
    • LAMA2
    纯化方法 Unpurified
    UniProt编号
    • P24043
    UniProt汇总 FUNCTION: SwissProt: P24043 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    SIZE: 3110 amino acids; 342771 Da
    SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (merosin) and laminin-4 (S- merosin).
    SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
    TISSUE SPECIFICITY: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
    DOMAIN: SwissProt: P24043 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular.
    DISEASE: SwissProt: P24043 # Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
    SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on Human Placenta lysate.

    Western Blot Analysis:
    1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB1922,Anti-Laminin α2 Antibody, clone 5H2

上一件merck millipore产品：merck millipore,默克密理博,655205,TWEEN 20 Detergent - CAS 9005-64-5 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,OR03L,Streptavidin, Horseradish Peroxidase Conjugate


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