merck millipore,默克密理博,MAB1676-C,Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free

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merck millipore,默克密理博,MAB1676-C,Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free
产品名称：Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free
产品型号：MAB1676-C
This Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free is validated for use in western blotting, IHC (Paraffin), IP, inhibition & immunofluorescence for the detection of Talin 1.

merck millipore,默克密理博,MAB1676-C,Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free

产品介绍

merck millipore,默克密理博,MAB1676-C,Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB1676-C
描述	Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free
Alternate Names	Talin-1
背景信息	Talin 1, also known as Talin 1, and encoded by the gene TLN1/KIAA1027, a large connecting protein located on the cytoplasmic side of most cellular projections. Talin appears to act as an anchoring protein connecting the cytoskeletal protein structures to the plasma membrane. Talin gets concentrated at regions of cell-substratum contact and in particular cells such as lymphocytes at regions of direct cell-cell contact. . Talin 1 binds with high affinity to vinculin and interacts with other proteins found in cellular projections including focal adhesion kinases (FAK) and intermediate filament proteins such as synemin and layilin. Additionally, Talin 1 because of its connections with cytoskeletal proteins talin is associated with many vital cell motion/growth processes from axon guidance to cell migration, muscle contraction, cell-cell interactions, immune responses and platelet activation to name but a few.

产品信息
格式	Purified
演示	Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

应用
应用	This Anti-Talin 1 Antibody, clone TA205, N-Term, Ascites Free is validated for use in western blotting, IHC (Paraffin), IP, inhibition & immunofluorescence for the detection of Talin 1.
主要应用	Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected Talin 1 in human prostate cancer sections and human lung tissue, respectively. Immunohistochemistry Analysis: A representative lot detected Talin 1 in MeOH fixed fibroblasts. Inhibition Analysis: A representative lot from an independent laboratory disrupts actin stress fibers and focal adhesions, and inhibits cell motility when microinjected into human fibroblasts (Bolton, S. J., et al. (1997). Cell Motil Cytoskeleton. 36(4): 363-376.). Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from shCTRL and shLRP-1-c1 carcinoma cell lysates (Dedieu, S., et al. (2008). Mol Cell Biol. 28(9):2980-2995.). Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from shCTRL and shLRP-1 cell lysates (Langlois, B., et al. (2010). PLoS One. 5(7):e11584.). Immunoprecipitation/Western Blotting Analysis: A representative lot from an independent laboratory immunoprecipitated Talin 1 from PC3-2 cell lysates. Immunoprecipitated Talin 1 was then detected in Western Blotting (Trerotola, M. et al. (2012). J Cell Physiol. 227(11): 3670-3676.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected Talin 1 in macrophages (Love, D. C., et al. (1998). Exp Parasitol. 88(3):161-171.). Immunofluorescence Analysis: A representative lot from an independent laboratory determined that the level of disruption in talin-containing focal complexes is inhibited in LRP-1-silenced carcinoma cells (Dedieu, S., et al. (2008). Mol Cell Biol. 28(9):2980-2995.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected Talin-1 in shCTRL and shLRP-1 transfected cells (Langlois, B., et al. (2010). PLoS One. 5(7):e11584.).

生物信息
免疫原品种	GST-tagged recombinant protein corresponding to the N-terminus of human Talin 1.
表位	N-terminus
克隆	TA205
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
同种型	IgG1κ
品种反应性	Human
Species Reactivity Note	Human. Predicted to react with Rabbit based on 100% sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_006280
基因符号	TLN TLN1
纯化方法	Protein G purified
UniProt编号	Q9Y490

产品使用声明
质量保证	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected Talin 1 in 10 µg of HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.