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描述 概述 Recombinant, human topoisomerase I fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Since Topo I can cause large amounts of single-strand DNA breaks it is an important target for antitumor agents. One unit= 1 ng protein. 产品目录编号 614850 品牌系列 Calbiochem®
同义词 Topo I, Wild Type 参考 参考 Pommier, Y., et al. 1998. Biochim. Biophys. Acta 1400, 83.
Pourquier, P., et al. 1997. J. Biol. Chem. 272, 26441.
Rossi, F., et al. 1996. Nature 381, 80.
Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 785.
Giovanella, B.C., et al. 1989. Science 246, 1046.
Wang, J,C. 1985. Annu. Rev. Biochem. 54, 665.产品信息 形式 Liquid 配方 5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0). Molar Mass 85000 生物信息 生物活性 0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction. 纯度 ≥95% by SDS-PAGE 特异活性 1 unit/ng protein 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Dry Ice Only
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).
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