merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1618,Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

  • merck millipore,默克密理博,MAB1618,Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

    产品名称：Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1
    产品型号：MAB1618
    Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 detects level of Dynein & has been published & validated for use in IC, IF, IP & WB.

    merck millipore,默克密理博,MAB1618,Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

  • 产品介绍
  • merck millipore,默克密理博,MAB1618,Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Xn, H, M, R, B, Dr, Sh, FWB, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB1618
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1
    Alternate Names
    • immotile cilia syndrome 1
    • Axonemal dynein intermediate chain 1
    • dynein intermediate chain DNAI1
    • dynein, axonemal, intermediate chain 1
    • dynein, axonemal, intermediate polypeptide 1
    • intermediate chain 1
    • intermediate polypeptide 1
    背景信息 Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These protein "motors" contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile funtions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kDa), three intermediate chains (70-125 kDa), and at least four light chains (15-25 kDa).
    产品信息
    格式 Purified
    控制
    • HeLa cells, A431 cell lysate
    演示 Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
    应用
    应用 Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 detects level of Dynein & has been published & validated for use in IC, IF, IP & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    应用说明 Immunocytochemistry:
    1:50-1:100 dilution from a previous lot was used. Reacts with cultured MDCK cells, NRR cells, N1E cells, and PTK-1 cells.

    Immunoprecipitation:
    10 μL of a previous lot per 0.5 gm tissue was used. The monoclonal cleanly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530 kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.

    Immunoblotting:
    1:1,000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Bovine brain cytoplasmic dynein.
    表位 74 kDa Intermediate chains, cytoplasmic
    克隆 74.1
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reacts with all known forms of the Mr = 74,000 Dalton intermediate chain subunit (IC74) of mammaliam cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes).
    同种型 IgG2b
    品种反应性 XenopusHumanMouseRatBovineDrosophilaSheepFish
    Species Reactivity Note Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid. Not tested on chicken, nematodes or Dictystelium.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_012144.2
    Entrez基因汇总 The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome.
    基因符号
    • CILD1
    • DNAI1
    • PCD
    • ICS
    • MGC26204
    非反应性品种
    • Squid
    纯化方法 Protein A purfied
    UniProt编号
    • Q0III3
    • Q29RQ3
    UniProt汇总 FUNCTION: SwissProt: Q9UI46 # Part of the dynein complex of respiratory cilia.
    SIZE: 699 amino acids; 79283 Da
    SUBUNIT: Consists of at least two heavy chains and a number of intermediate and light chains.
    DISEASE: SwissProt: Q9UI46 # Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:242650]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. & Defects in DNAI1 are a cause of Kartagener syndrome [MIM:244400]. Kartagener syndrome is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus.
    SIMILARITY: SwissProt: Q9UI46 ## Belongs to the dynein intermediate chain family. & Contains 5 WD repeats.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on A431 lysates.

    Western Blot Analysis:
    1:500 dilution of this lot detected DYNEIN on 10 μg of A431 lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB1618,Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1

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