merck millipore,默克密理博,04-1479,Anti-methyl-PP2A Antibody, C subunit, clone 2A10

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merck millipore,默克密理博,04-1479,Anti-methyl-PP2A Antibody, C subunit, clone 2A10
产品名称：Anti-methyl-PP2A Antibody, C subunit, clone 2A10
产品型号：04-1479
This Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.

merck millipore,默克密理博,04-1479,Anti-methyl-PP2A Antibody, C subunit, clone 2A10

产品介绍

merck millipore,默克密理博,04-1479,Anti-methyl-PP2A Antibody, C subunit, clone 2A10

Replacement Information
Replacement Information	04-1479 is a recommended replacement for 05-546.

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Dr, H, M, Yeast (S. cerevisiae), R, Po, Rb, Ch	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	04-1479
Replaces	05-546
描述	Anti-methyl-PP2A Antibody, C subunit, clone 2A10
背景信息	Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

产品信息
格式	Purified
控制	Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
演示	Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.

应用
应用	This Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.
克隆	2A10
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes the methylated form of PP2A, catalytic subunit.
同种型	IgG1κ
品种反应性	DrosophilaHumanMouseYeast (S. cerevisiae)RatPigRabbitChicken
Species Reactivity Note	Human, mouse and yeast. Predicted to cross react with rat, pig, rabbit, chicken and Drosophila.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002706.1
修改	Methylation
纯化方法	Protein G purfied
UniProt编号	P67775

产品使用声明
质量保证	Evaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1. Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Evaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.

Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.