- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M, Po WB, ICC, IHC M Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MAB1527 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Peripherin Antibody, clone 8G2 背景信息 Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration. 产品信息 格式 Culture Supernatant 控制 - Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate
演示 UnPurified mouse culture supernatant containing no preservatives. 应用 应用 Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
不建议的应用 - Immunohistochemistry (Paraffin)
应用说明 Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.
Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200
Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE 克隆 8G2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin. 同种型 IgG 品种反应性 RatBovineHumanMousePig 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_006262.3
Entrez基因汇总 This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. 基因符号 - NEF4
- PRPH
- PRPH1
- Peripherin.
- peripherin
纯化方法 Unpurified UniProt编号 - P41219
UniProt汇总 FUNCTION: SwissProt: P41219 # Class-III neuronal intermediate filament protein.
SIZE: 470 amino acids; 53651 Da
SIMILARITY: SwissProt: P41219 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 300 µL
上一件merck millipore产品：merck millipore,默克密理博,ABC822,Anti-LIMP II Antibody
下一件merck millipore产品：merck millipore,默克密理博,524748,PDE4D Inhibitor, GEBR-7b - Calbiochem
相关产品
merck millipore,默克密理博,495609,InSolution™ Okadaic Acid, Prorocentrum sp. - Calbiochem
merck millipore,默克密理博,71724,pIEx/Bac™-1 DNA - Novagen
merck millipore,默克密理博,S26-KC,Goat Serum (1L) KC
merck millipore,默克密理博,ABC822,Anti-LIMP II Antibody
merck millipore,默克密理博,524748,PDE4D Inhibitor, GEBR-7b - Calbiochem
merck millipore,默克密理博,05-1149,Anti-ADP-Ribosylation Factor 6 Antibody, clone 6ARF01
merck millipore,默克密理博,416154,Pre-mRNA Splicing Inhibitor, Isoginkgetin - CAS 548-19-6 - Calbiochem
merck millipore,默克密理博,100107,A23187, 4-Bromo- - CAS 76455-82-8 - Calbiochem
merck millipore,默克密理博,AB3081,Anti-Na+/H+ Exchanger-1 Antibody