merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1510,Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

  • merck millipore,默克密理博,MAB1510,Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

    产品名称：Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)
    产品型号：MAB1510
    This Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS) is validated for use in IH(P), IP, WB for the detection of Ubiquitin.

    merck millipore,默克密理博,MAB1510,Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

  • 产品介绍
  • merck millipore,默克密理博,MAB1510,Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWBMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB1510
    Replaces 04-454
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)
    Alternate Names
    • ubiquitin-CEP80
    • Ubiquitin
    背景信息 Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
    产品信息
    格式 Ascites
    控制
    • Alzheimer's diseased brain.
    演示 Mouse monoclonal IgG1κ in buffer containing containing 10 mM sodium azide.
    应用
    应用 This Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS) is validated for use in IH(P), IP, WB for the detection of Ubiquitin.
    主要应用
    • Western Blotting
    应用说明 Immunohistochemistry (frozen or paraffin embedded sections):
    1:250-1:1,000 dilution from a previous lot was used.

    Electron Microscopy:
    1:250-1:1,000 dilution from a previous lot was used.

    Immunoprecipitation:
    (Liang, 1995; Oberdorf, 1999)
    Note: Suggested blocking agent is bovine serum albumin.

    Immunoblotting:
    1:250-1:1,000. The sensitivity of the assay can be enhanced by autoclaving the blot (Swerdlow, 1986).

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Purified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde.
    克隆 Ubi-1 (aka 042691GS)
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Ubiquitin (76 amino acid, 8.5 kDa) both conjugated and unconjugated. Shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson’s disease) and Pick bodies (in Pick’s disease).
    同种型 IgG1κ
    品种反应性 RatHumanMouse
    Species Reactivity Note Expected to cross- react with Bovine, Chicken, and Drosophila. Reactivity with other species has not been confirmed.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_002954.3
    Entrez基因汇总 This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
    基因符号
    • UBCEP2
    • RPS27A
    • UBA80
    • UBC
    • CEP80
    • Uba80
    • UBCEP80
    • UBCEP1:UBA52
    • UBB
    • HUBCEP80
    纯化方法 Unpurified
    UniProt编号
    • P62988
    UniProt汇总 FUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
    SIZE: 76 amino acids; 8565 Da
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
    PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
    SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
    MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on Jurkat lysates.

    Western Blot Analysis:
    1:500 dilution of this lot detected UBIQUITIN on 10 μg of Jurkat3 lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C in undiluted aliquotes from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB1510,Anti-Ubiquitin Antibody, clone Ubi-1 (aka 042691GS)

上一件merck millipore产品：merck millipore,默克密理博,12-359,Acetyl-Histone H3 (Lys14) Peptide
下一件merck millipore产品：merck millipore,默克密理博,MABF83,Anti-CD2 Antibody, clone RPA-2.10


相关产品

merck millipore,默克密理博,AB9896,Anti-ACE2 Antibody merck millipore,默克密理博,AB9896,Anti-ACE2 Antibody

merck millipore,默克密理博,614310,TLR3/dsRNA Complex Inhibitor - Calbiochem merck millipore,默克密理博,614310,TLR3/dsRNA Complex Inhibitor - Calbiochem

merck millipore,默克密理博,AG938-1MG,Synuclein, α, recombinant human merck millipore,默克密理博,AG938-1MG,Synuclein, α, recombinant human

merck millipore,默克密理博,TMS-006-C,EmbryoMax Ultra Pure Water, sterile H2O, 100ml merck millipore,默克密理博,TMS-006-C,EmbryoMax Ultra Pure Water, sterile H2O, 100ml

merck millipore,默克密理博,12-359,Acetyl-Histone H3 (Lys14) Peptide merck millipore,默克密理博,12-359,Acetyl-Histone H3 (Lys14) Peptide

merck millipore,默克密理博,MABF83,Anti-CD2 Antibody, clone RPA-2.10 merck millipore,默克密理博,MABF83,Anti-CD2 Antibody, clone RPA-2.10

merck millipore,默克密理博,ABT349,Anti-EFS Antibody merck millipore,默克密理博,ABT349,Anti-EFS Antibody

merck millipore,默克密理博,200100,Benzyl-2-acetamido-2-deoxy-α-D-galactopyranoside - CAS 3554-93-6 - Calbiochem merck millipore,默克密理博,200100,Benzyl-2-acetamido-2-deoxy-α-D-galactopyranoside - CAS 3554-93-6 - Calbioc...

merck millipore,默克密理博,420640,Ketohexokinase Inhibitor - Calbiochem merck millipore,默克密理博,420640,Ketohexokinase Inhibitor - Calbiochem

merck millipore,默克密理博,DPP4,DPP IV Inhibitor merck millipore,默克密理博,DPP4,DPP IV Inhibitor


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部