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品种反应性 主要应用 宿主 格式 抗体类型 H, Ch WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 MAB13435 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-2 Antibody, clone 7F3.2 Alternate Names - 72 kDa Type IV Collagenase
- Gelatinase A
产品信息 格式 Purified 演示 Liquid at 1 mg/mL containing sodium azide as a preservative. 应用 应用 Anti-MMP-2 Antibody, clone 7F3.2 is an antibody against MMP-2 for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Ideal for Western Blotting and EIA applications. When used against the reduced protein, it identifies both a 72 kDa and a 68 kDa species (the pro-form and active form of the enzyme, respectively). Other applications have not been evaluated.
Gelatinase-A is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen . The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Recommended antibody dilution for Western Blots is 1:1,000 when using colorimetric substrates such BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be required for non-human samples.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide based on the human sequence. 克隆 7F3.2 宿主 Mouse 特异性 Binds to Gelatinase-A. No cross reactivity with the other MMP family members (MMP-1, MMP-2B, MMP-3, and MMP-9). 同种型 IgG1 品种反应性 HumanChicken 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_004530.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. 基因符号 - EC 3.4.24.24
- MMP2
- TBE-1
- CLG4A
- CLG4
- MMP-II
- MONA
- MMP-2
UniProt编号 - P08253
UniProt汇总 FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 660 amino acids; 73882 Da
SUBUNIT: Ligand for integrin alpha-V/beta-3.
TISSUE SPECIFICITY: Produced by normal skin fibroblasts.
DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).
DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The undiluted antibody solution is stable for approximately 6 months when stored -20°C. 包装信息 数量 100 µg
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