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检测方法 Fluorometric 描述 概述 A convenient assay for chemotaxis cell migration in 24-well plates. Cells migrate through a 8 μm pore to a feeder layer containing serum or other attractant. Migrated cells are quantitated using a cell-permeable fluorescent dye. 产品目录编号 CBA017 品牌系列 Calbiochem®
参考 参考 Wells, A., et al. 2002. Acta Oncol. 41, 124.
Staff, A. C. 2001. Scand. J. Clin. Lab. Invest. 61, 257.
Eatock, M.M., et al. 2000. Cancer Treat. Rev. 26, 19.
Klinghoffer, R.A., et al. 1999. EMBO J. 18, 2459.
Sieg, D.J., et al. 1999. J. Cell Sci. 112, 2677.
Smilenov, L. B., et al. 1999. Science 286, 1172.
Burridge, K. et al. 1997. Trends Cell Biol. 7, 342.
Huttenlocher, A., et al. 1997. J. Biol. Chem. 272, 32719.
Price, J.T., et al. 1997. Crit. Rev. Biochem. Mol. Biol. 32, 175.
Lauffenburger, D.A., and Horowitz, A.F. 1996. Cell 84, 359.产品信息 检测方法 Fluorometric 形式 12 Tests 格式 24-well plate 试剂盒包含 12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol. 生物信息 化验时间 3-5 h 样品类型 Adherent cells 理化信息 最大排放 ~520 nm 最大激励 ~485 nm 产品使用声明 预定用途 The InnoCyte™ Cell Migration Assay, 24-Well is suited for studying the effects of various drugs or agents on cell motility and for identifying chemoattractant agents. The Cell Culture Inserts have an 8-µm pore size membrane that is suitable for migration of epithelial, mesenchymal, and endothelial cell types. The membrane is not coated with an extracellular matrix protein, so is not suitable for haptotactic migration studies. The assay is not intended for leukocyte migration experiments; leucocytes require membranes with a pore size that is smaller than 8 µm. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C. 避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 12 Sterile Cell Culture Inserts, Sterile 24-well Plate, Black Plate Strips, Cell Detachment Buffer, Fluorescent Dye, Latrunculin A (inhibitor), and a user protocol.
上一件merck millipore产品：merck millipore,默克密理博,IHCR2025-6,IHC Select Anti-Cytokeratin AE1/AE3 (Pan cytokeratins) Antibody, prediluted, clone AE1/AE3
下一件merck millipore产品：merck millipore,默克密理博,MABC006,Mouse IgG2b Negative Control, clone GC198
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