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merck millipore,默克密理博,MAB1285FX,Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated
产品名称：Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated
产品型号：MAB1285FX
Detect HLA-B27 using this Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated validated for use in FC.
merck millipore,默克密理博,MAB1285FX,Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC M FITC Monoclonal Antibody 描述 产品目录编号 MAB1285FX 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated 概述 BIOCHEMISTRY: pI = 8.0 产品信息 格式 FITC 演示 Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in 1 ml phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry. 应用 应用 Detect HLA-B27 using this Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated validated for use in FC. 主要应用 - Flow Cytometry
应用说明 This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
Flow cytometry and immunofluorescence 1:15 to 1:60
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.生物信息 免疫原品种 Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A. 克隆 HLA.ABC.m3 宿主 Mouse 特异性 This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7 (9.5x107M-). 同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005514.5
Entrez基因汇总 HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. 基因符号 - Bw-44
- HLA-B
- Bw-54
- Bw-45
- Bw-47
- Bw-46
- Bw-52
- B-5
- Bw-4
- Bw-57
- OTTHUMP00000034826
- AS
- Bw-56
- Bw-55
- Bw-41
- Bw-48
- B'DT
- Bw-50
- Bw-60
- Bw-58
- B-21
- HLA-B-5501
- B-12
- Bw-22
- Bw-53
- HLAB
- SPDA1
UniProt编号 - P03989
UniProt汇总 FUNCTION: SwissProt: P30498 # Involved in the presentation of foreign antigens to the immune system.| Q31612 # Involved in the presentation of foreign antigens to the immune system.| P30488 # Involved in the presentation of foreign antigens to the immune system.| P03989 # Involved in the presentation of foreign antigens to the immune system.| P30493 # Involved in the presentation of foreign antigens to the immune system.| P30460 # Involved in the presentation of foreign antigens to the immune system.| P30484 # Involved in the presentation of foreign antigens to the immune system.| P01889 # Involved in the presentation of foreign antigens to the immune system.| P30485 # Involved in the presentation of foreign antigens to the immune system.| Q29940 # Involved in the presentation of foreign antigens to the immune system.| P30483 # Involved in the presentation of foreign antigens to the immune system.| P30495 # Involved in the presentation of foreign antigens to the immune system.| P30492 # Involved in the presentation of foreign antigens to the immune system.| P18465 # Involved in the presentation of foreign antigens to the immune system.| P30487 # Involved in the presentation of foreign antigens to the immune system.| P30480 # Involved in the presentation of foreign antigens to the immune system.| P30491 # Involved in the presentation of foreign antigens to the immune system.| Q95365 # Involved in the presentation of foreign antigens to the immune system.| Q29836 # Involved in the presentation of foreign antigens to the immune system.| Q29718 # Involved in the presentation of foreign antigens to the immune system.| P30475 # Involved in the presentation of foreign antigens to the immune system.| P18464 # Involved in the presentation of foreign antigens to the immune system.| P30479 # Involved in the presentation of foreign antigens to the immune system.| P30490 # Involved in the presentation of foreign antigens to the immune system.| P10319 # Involved in the presentation of foreign antigens to the immune system.| P30466 # Involved in the presentation of foreign antigens to the immune system.| P30461 # Involved in the presentation of foreign antigens to the immune system.| P18463 # Involved in the presentation of foreign antigens to the immune system.| Q04826 # Involved in the presentation of foreign antigens to the immune system.| P30481 # Involved in the presentation of foreign antigens to the immune system.| P30685 # Involved in the presentation of foreign antigens to the immune system.| P30462 # Involved in the presentation of foreign antigens to the immune system.| Q31610 # Involved in the presentation of foreign antigens to the immune system.| P30486 # Involved in the presentation of foreign antigens to the immune system.| P30464 # Involved in the presentation of foreign antigens to the immune system.
SIZE: 362 amino acids; 40478 Da
SUBUNIT: Heterodimer of an alpha chain and a beta chain (beta-2- microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P30498
PTM: Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).
DISEASE: "SwissProt: P03989 # HLA-B27 is associated with the development of ankylosing spondylitis (AS) [MIM:106300]. AS is a chronic inflammatory rheumatic disease that mainly affects the axial skeleton and is considered the prototype of seronegative spondyloarthropathies (SNSA), which include reactive arthritis (e.g., Reiter's syndrome), psoritic spondylitis, and other spondyloarthropathies (SpA). In the Greek Cypriot population, a restricted number of HLA-B27 subtypes are associated with AS and other B27-related diseases and an elevated frequency of the B*2702 allele in the AS patients is identified. The allele B*2707 seems to have a protective role in this population because it was found only in the healthy controls.| P18464 # HLA-B51 is associated with Behcet disease but it is not certain whether HLA-B51 itself or a closely linked gene is responsible for susceptibility. About 20% of healthy individuals of various ethnic origin carries the HLA-B 51 locus, compared with 50 to 80% of patients. Behcet disease is a systemic disorder of recurrent acute inflammation characterized by 4 major symptoms: oral aphthous ulcers, skin lesions, ocular symptoms and genital ulcerations. Occasionally, inflammation in tissues and organs throughout the body can occur, including the gastrointestinal tract, central nervous system, vascular system, lungs, and kidneys. The etiology of Behcet disease is unclear.| P30464 # Involved in susceptibility to Stevens-Johnson syndrome [MIM:608579]. This is a life-threatening reaction of the skin to particular types of medication. It is characterized by high fever, malaise and blistering exanthema. The more severe form of the disease is referred to as toxic epidermal necrolysis."
SIMILARITY: SwissProt: P30498 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31612 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30488 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P03989 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30493 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30460 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30484 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P01889 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30485 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29940 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30483 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30495 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30492 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18465 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30487 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30480 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30491 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q95365 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29836 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q29718 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30475 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30479 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30490 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P10319 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30466 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30461 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P18463 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q04826 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30481 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30685 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30462 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | Q31610 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30486 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain. | P30464 ## Belongs to the MHC class I family. & Contains 1 Ig-like C1-type (immunoglobulin-like) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2 to 8°C. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.包装信息 数量 100 assays
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